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Journal of Virology, August 2008, p. 7306-7312, Vol. 82, No. 15
0022-538X/08/$08.00+0     doi:10.1128/JVI.00512-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Visualization of the Externalized VP2 N Termini of Infectious Human Parvovirus B19 {triangledown}

Bärbel Kaufmann,1 Paul R. Chipman,1 Victor A. Kostyuchenko,1 Susanne Modrow,2 and Michael G. Rossmann1*

Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907-2054,1 Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany2

Received 7 March 2008/ Accepted 16 May 2008

The structures of infectious human parvovirus B19 and empty wild-type particles were determined by cryoelectron microscopy (cryoEM) to 7.5-Å and 11.3-Å resolution, respectively, assuming icosahedral symmetry. Both of these, DNA filled and empty, wild-type particles contain a few copies of the minor capsid protein VP1. Comparison of wild-type B19 with the crystal structure and cryoEM reconstruction of recombinant B19 particles consisting of only the major capsid protein VP2 showed structural differences in the vicinity of the icosahedral fivefold axes. Although the unique N-terminal region of VP1 could not be visualized in the icosahedrally averaged maps, the N terminus of VP2 was shown to be exposed on the viral surface adjacent to the fivefold β-cylinder. The conserved glycine-rich region is positioned between two neighboring, fivefold-symmetrically related VP subunits and not in the fivefold channel as observed for other parvoviruses.

* Corresponding author. Mailing address: Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907-2054. Phone: (765) 494-4911. Fax: (765) 496-1189. E-mail: mr{at}purdue.edu

{triangledown} Published ahead of print on 28 May 2008.

Journal of Virology, August 2008, p. 7306-7312, Vol. 82, No. 15
0022-538X/08/$08.00+0     doi:10.1128/JVI.00512-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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Copyright © 2008 by the American Society for Microbiology. All rights reserved.