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Journal of Virology, July 2008, p. 7238-7242, Vol. 82, No. 14
0022-538X/08/$08.00+0     doi:10.1128/JVI.00425-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cell Adhesion Promotes Ebola Virus Envelope Glycoprotein-Mediated Binding and Infection{triangledown}

Derek Dube,1 Kathryn L. Schornberg,1 Tzanko S. Stantchev,3 Matthew I. Bonaparte,3 Sue E. Delos,2 Amy H. Bouton,1 Christopher C. Broder,3 and Judith M. White1,2*

Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave., Charlottesville, Virginia 22908-0734,1 Department of Cell Biology, University of Virginia, 1300 Jefferson Park Ave., Charlottesville, Virginia 22908-0734,2 Department of Microbiology and Immunology, Uniformed Services University, Bethesda, Maryland 208143

Received 26 February 2008/ Accepted 22 April 2008

Ebola virus infects a wide variety of adherent cell types, while nonadherent cells are found to be refractory. To explore this correlation, we compared the ability of pairs of related adherent and nonadherent cells to bind a recombinant Ebola virus receptor binding domain (EboV RBD) and to be infected with Ebola virus glycoprotein (GP)-pseudotyped particles. Both human 293F and THP-1 cells can be propagated as adherent or nonadherent cultures, and in both cases adherent cells were found to be significantly more susceptible to both EboV RBD binding and GP-pseudotyped virus infection than their nonadherent counterparts. Furthermore, with 293F cells the acquisition of EboV RBD binding paralleled cell spreading and did not require new mRNA or protein synthesis.

* Corresponding author. Mailing address: Department of Cell Biology, University of Virginia, 1300 Jefferson Park Ave., Charlottesville, VA 22908-0734. Phone: (434) 924-2593. Fax: (434) 982-3912. E-mail: jw7g{at}virginia.edu

{triangledown} Published ahead of print on 30 April 2008.

Journal of Virology, July 2008, p. 7238-7242, Vol. 82, No. 14
0022-538X/08/$08.00+0     doi:10.1128/JVI.00425-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schornberg, K. L., Shoemaker, C. J., Dube, D., Abshire, M. Y., Delos, S. E., Bouton, A. H., White, J. M. (2009). {alpha}5{beta}1-Integrin controls ebolavirus entry by regulating endosomal cathepsins. Proc. Natl. Acad. Sci. USA 106: 8003-8008 [Abstract] [Full Text]  
  • Dube, D., Brecher, M. B., Delos, S. E., Rose, S. C., Park, E. W., Schornberg, K. L., Kuhn, J. H., White, J. M. (2009). The Primed Ebolavirus Glycoprotein (19-Kilodalton GP1,2): Sequence and Residues Critical for Host Cell Binding. J. Virol. 83: 2883-2891 [Abstract] [Full Text]  


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