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Journal of Virology, July 2008, p. 6962-6971, Vol. 82, No. 14
0022-538X/08/$08.00+0     doi:10.1128/JVI.00133-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus Inhibits Cell Cytokinesis and Proliferation by Interacting with Translation Elongation Factor 1{alpha} {triangledown}

Bing Zhou,{dagger} Junli Liu,{dagger} Qiuna Wang, Xuan Liu, Xiaorong Li, Ping Li, Qingjun Ma, and Cheng Cao*

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China

Received 18 January 2008/ Accepted 24 April 2008

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of SARS, an emerging disease characterized by atypical pneumonia. Using a yeast two-hybrid screen with the nucleocapsid (N) protein of SARS-CoV as a bait, the C terminus (amino acids 251 to 422) of the N protein was found to interact with human elongation factor 1-alpha (EF1{alpha}), an essential component of the translational machinery with an important role in cytokinesis, promoting the bundling of filamentous actin (F-actin). In vitro and in vivo interaction was then confirmed by immuno-coprecipitation, far-Western blotting, and surface plasmon resonance. It was demonstrated that the N protein of SARS-CoV induces aggregation of EF1{alpha}, inhibiting protein translation and cytokinesis by blocking F-actin bundling. Proliferation of human peripheral blood lymphocytes and other human cell lines was significantly inhibited by the infection of recombinant retrovirus expressing SARS-CoV N protein.

* Corresponding author. Mailing address: Beijing Institute of Biotechnology, P.O. Box 130(8), 27 Taiping Rd., Beijing 100850, China. Phone: 86 10 6815 5151. Fax: 86 10 63853882. E-mail: caoc{at}nic.bmi.ac.cn

{triangledown} Published ahead of print on 30 April 2008.

{dagger} B.Z. and J.L. contributed equally to this work.

Journal of Virology, July 2008, p. 6962-6971, Vol. 82, No. 14
0022-538X/08/$08.00+0     doi:10.1128/JVI.00133-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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