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Journal of Virology, June 2008, p. 5390-5397, Vol. 82, No. 11
0022-538X/08/$08.00+0     doi:10.1128/JVI.00094-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Proteomic Analysis of Pathogenic and Attenuated Alcelaphine Herpesvirus 1{triangledown}

Inga Dry,1,{dagger} David M. Haig,1,{ddagger} Neil F. Inglis,1 Lisa Imrie,1 James P. Stewart,2 and George C. Russell1*

Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, Scotland,1 School of Infection and Host Defence, University of Liverpool, Liverpool, United Kingdom2

Received 15 January 2008/ Accepted 12 March 2008

The gammaherpesvirus alcelaphine herpesvirus 1 (AlHV-1) causes malignant catarrhal fever in susceptible ungulates but infects its natural host, wildebeest, without obvious clinical signs. In tissue culture, AlHV-1 is initially predominantly cell associated and virulent but on extended culture becomes cell-free and attenuated. We wanted to determine what changes in protein composition had taken place during the transition from virulent to attenuated virus in culture. Purified virus preparations were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and proteins were analyzed by liquid chromatography-electrospray ionization-tandem mass spectrometry. Peptides were identified in serial gel slices by using MASCOT software to interrogate virus-specific and nonredundant sequence databases. Twenty-three AlHV-1-encoded proteins and six cellular proteins were identified in the attenuated and virulent viruses. Two polypeptides were detected in only the virulent virus preparations, while one other protein was found in only the attenuated virus. Two of these virus-specific proteins were identified by a single peptide, suggesting that these may be low-abundance virion proteins rather than markers of attenuation or pathogenesis. The results suggest that attenuation of AlHV-1 is not the result of gross changes in the composition of the virus particle but probably due to altered viral gene expression in the infected cell.

* Corresponding author. Mailing address: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, United Kingdom. Phone: 44 131 445 5111. Fax: 44 131 445 6111. E-mail: George.Russell{at}moredun.ac.uk

{triangledown} Published ahead of print on 19 March 2008.

{dagger} Present address: Centre for Infectious Disease, Barts and The London School of Medicine and Dentistry, London, United Kingdom.

{ddagger} Present address: School of Veterinary Medicine and Science, Nottingham University, Nottingham, United Kingdom.

Journal of Virology, June 2008, p. 5390-5397, Vol. 82, No. 11
0022-538X/08/$08.00+0     doi:10.1128/JVI.00094-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Loret, S., Guay, G., Lippe, R. (2008). Comprehensive Characterization of Extracellular Herpes Simplex Virus Type 1 Virions. J. Virol. 82: 8605-8618 [Abstract] [Full Text]  


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