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Journal of Virology, January 2008, p. 582-587, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.01451-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification and Classification of Endogenous Retroviruses in Cattle{triangledown} ,{dagger}

Rui Xiao, Kwangha Park, Hoontaek Lee, Jinhoi Kim, and Chankyu Park*

Laboratory of Mammalian Genomics, Department of Animal Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea

Received 3 July 2007/ Accepted 15 October 2007

The aim of this study was to identify the endogenous retrovirus (ERV) sequences in a bovine genome. We subjected bovine genomic DNA to PCR with degenerate or ovine ERV (OERV) family-specific primers that aimed to amplify the retroviral pro/pol region. Sequence analysis of 113 clones obtained by PCR revealed that 69 were of retroviral origin. On the basis of the OERV classification system, these clones from degenerate PCR could be divided into the β3, {gamma}4, and {gamma}9 families. PCR with OERV family-specific primers revealed an additional ERV that was classified into the bovine endogenous retrovirus (BERV) {gamma}7 family. In conclusion, here we report the results of a genome scale study of the BERV. Our study shows that the ERV family expansion in cattle may be somewhat limited, while more diverse family members of ERVs have been reported from other artiodactyls, such as pigs and sheep.

* Corresponding author. Mailing address: Department of Animal Biotechnology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 143-701, South Korea. Phone: 82-2-450-3697. Fax: 82-2-457-8488. E-mail: chankyu{at}konkuk.ac.kr

{triangledown} Published ahead of print on 24 October 2007.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, January 2008, p. 582-587, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.01451-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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