This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Valentine, L. E.
Right arrow Articles by Watkins, D. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Valentine, L. E.
Right arrow Articles by Watkins, D. I.

 Previous Article  |  Next Article 

Journal of Virology, January 2008, p. 575-581, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.00275-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Recognition of Escape Variants in ELISPOT Does Not Always Predict CD8+ T-Cell Recognition of Simian Immunodeficiency Virus-Infected Cells Expressing the Same Variant Sequences{triangledown}

Laura E. Valentine,1 Shari M. Piaskowski,2 Eva G. Rakasz,1 Nathan L. Henry,1 Nancy A. Wilson,1 and David I. Watkins1,2*

Wisconsin National Primate Research Center,1 Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin2

Received 8 February 2007/ Accepted 12 October 2007

Human immunodeficiency virus (HIV)'s tremendous sequence variability is a major obstacle for the development of cytotoxic-T-lymphocyte-based vaccines, especially since much of this variability is selected for by CD8+ T cells. We investigated to what extent reactivity to escape variant peptides in standard enzyme-linked immunospot (ELISPOT) assays predicts the recognition of cells infected with corresponding escape variant viruses. Most of the variant peptides tested were recognized in standard ELISPOT and intracellular cytokine stain (ICS) assays. Functional avidity of epitope-specific T cells for some of the variants was, however, markedly reduced. These mutations which reduced avidity also abrogated recognition by epitope-specific CD8+ T cells in a viral suppression assay. Our results indicate that "cross-reactive" CD8+ T-cell responses identified in ELISPOT and ICS assays using a single high concentration of variant peptide often fail to predict the recognition of cells infected with variant viruses.

* Corresponding author. Mailing address: 555 Science Dr., Madison, WI 53711. Phone: (608) 265-3380. Fax: (608) 265-8084. E-mail: watkins{at}primate.wisc.edu

{triangledown} Published ahead of print on 24 October 2007.

Journal of Virology, January 2008, p. 575-581, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.00275-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Korber, B. T., Letvin, N. L., Haynes, B. F. (2009). T-Cell Vaccine Strategies for Human Immunodeficiency Virus, the Virus with a Thousand Faces. J. Virol. 83: 8300-8314 [Full Text]  
  • Loffredo, J. T., Sidney, J., Bean, A. T., Beal, D. R., Bardet, W., Wahl, A., Hawkins, O. E., Piaskowski, S., Wilson, N. A., Hildebrand, W. H., Watkins, D. I., Sette, A. (2009). Two MHC Class I Molecules Associated with Elite Control of Immunodeficiency Virus Replication, Mamu-B*08 and HLA-B*2705, Bind Peptides with Sequence Similarity. J. Immunol. 182: 7763-7775 [Abstract] [Full Text]  
  • Goonetilleke, N., Liu, M. K.P., Salazar-Gonzalez, J. F., Ferrari, G., Giorgi, E., Ganusov, V. V., Keele, B. F., Learn, G. H., Turnbull, E. L., Salazar, M. G., Weinhold, K. J., Moore, S., CHAVI Clinical Core B, , Letvin, N., Haynes, B. F., Cohen, M. S., Hraber, P., Bhattacharya, T., Borrow, P., Perelson, A. S., Hahn, B. H., Shaw, G. M., Korber, B. T., McMichael, A. J. (2009). The first T cell response to transmitted/founder virus contributes to the control of acute viremia in HIV-1 infection. JEM 206: 1253-1272 [Abstract] [Full Text]  
  • Chen, H., Piechocka-Trocha, A., Miura, T., Brockman, M. A., Julg, B. D., Baker, B. M., Rothchild, A. C., Block, B. L., Schneidewind, A., Koibuchi, T., Pereyra, F., Allen, T. M., Walker, B. D. (2009). Differential Neutralization of Human Immunodeficiency Virus (HIV) Replication in Autologous CD4 T Cells by HIV-Specific Cytotoxic T Lymphocytes. J. Virol. 83: 3138-3149 [Abstract] [Full Text]  
  • Maness, N. J., Yant, L. J., Chung, C., Loffredo, J. T., Friedrich, T. C., Piaskowski, S. M., Furlott, J., May, G. E., Soma, T., Leon, E. J., Wilson, N. A., Piontkivska, H., Hughes, A. L., Sidney, J., Sette, A., Watkins, D. I. (2008). Comprehensive Immunological Evaluation Reveals Surprisingly Few Differences between Elite Controller and Progressor Mamu-B*17-Positive Simian Immunodeficiency Virus-Infected Rhesus Macaques. J. Virol. 82: 5245-5254 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»