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Journal of Virology, January 2008, p. 487-494, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.01875-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Temperature-Sensitive Mutations in the Putative Herpes Simplex Virus Type 1 Terminase Subunits pUL15 and pUL33 Preclude Viral DNA Cleavage/Packaging and Interaction with pUL28 at the Nonpermissive Temperature{triangledown}

Kui Yang,1 Alice P. W. Poon,2 Bernard Roizman,2 and Joel D. Baines1*

C5132 Veterinary Education Center, Department of Microbiology and Immunology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853,1 The Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Chicago, Illinois 606372

Received 27 August 2007/ Accepted 24 September 2007

Terminases comprise essential components of molecular motors required to package viral DNA into capsids in a variety of DNA virus systems. Previous studies indicated that the herpes simplex virus type 1 UL15 protein (pUL15) interacts with the pUL28 moiety of a pUL28-pUL33 complex to form the likely viral terminase. In the current study, a novel temperature-sensitive mutant virus was shown to contain a mutation in UL33 codon 61 predicted to change threonine to proline. At the nonpermissive temperature, this virus, designated ts8-22, replicated viral DNA and produced capsids that became enveloped at the inner nuclear membrane but failed to form plaques or to cleave or package viral DNA. Incubation at the nonpermissive temperature also precluded coimmunoprecipitation of UL33 protein with its normal interaction partners encoded by UL28 and UL15 in ts8-22-infected cells and with pUL28 in transient-expression assays. Moreover, a temperature-sensitive mutation in UL15 precluded coimmunoprecipitation of pUL15 with the UL28 and UL33 proteins at the nonpermissive temperature. We conclude that interactions between putative terminase components are tightly linked to successful viral DNA cleavage and packaging.

* Corresponding author. Mailing address: C5132 Veterinary Education Center, Department of Microbiology and Immunology, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. Phone: (607) 253-3391. Fax: (607) 253-3384. E-mail: jdb11{at}cornell.edu

{triangledown} Published ahead of print on 3 October 2007.

Journal of Virology, January 2008, p. 487-494, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.01875-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Beilstein, F., Higgs, M. R., Stow, N. D. (2009). Mutational Analysis of the Herpes Simplex Virus Type 1 DNA Packaging Protein UL33. J. Virol. 83: 8938-8945 [Abstract] [Full Text]  
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  • Loret, S., Guay, G., Lippe, R. (2008). Comprehensive Characterization of Extracellular Herpes Simplex Virus Type 1 Virions. J. Virol. 82: 8605-8618 [Abstract] [Full Text]  


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