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Journal of Virology, January 2008, p. 461-470, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.01894-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effect of Cell Polarization on Hepatitis C Virus Entry{triangledown}

Christopher J. Mee, Joe Grove, Helen J. Harris, Ke Hu, Peter Balfe,* and Jane A. McKeating

Division of Immunity and Infection, Institute for Biomedical Research, University of Birmingham, Birmingham, United Kingdom

Received 30 August 2007/ Accepted 12 October 2007

The primary reservoir for hepatitis C virus (HCV) replication in vivo is believed to be hepatocytes within the liver. Three host cell molecules have been reported to be important entry factors for receptors for HCV: the tetraspanin CD81, scavenger receptor BI (SR-BI), and the tight-junction (TJ) protein claudin 1 (CLDN1). The recent discovery of a TJ protein as a critical coreceptor highlighted the importance of studying the effect(s) of TJ formation and cell polarization on HCV entry. The colorectal adenocarcinoma Caco-2 cell line forms polarized monolayers containing functional TJs and was found to express the CD81, SR-BI, and CLDN1 proteins. Viral receptor expression levels increased upon polarization, and CLDN1 relocalized from the apical pole of the lateral cell membrane to the lateral cell-cell junction and basolateral domains. In contrast, expression and localization of the TJ proteins ZO-1 and occludin 1 were unchanged upon polarization. HCV infected polarized and nonpolarized Caco-2 cells to comparable levels, and entry was neutralized by anti-E2 monoclonal antibodies, demonstrating glycoprotein-dependent entry. HCV pseudoparticle infection and recombinant HCV E1E2 glycoprotein interaction with polarized Caco-2 cells occurred predominantly at the apical surface. Disruption of TJs significantly increased HCV entry. These data support a model where TJs provide a physical barrier for viral access to receptors expressed on lateral and basolateral cellular domains.

* Corresponding author. Mailing address: Division of Immunity and Infection, Institute for Biomedical Research, University of Birmingham, Vincent Drive, Birmingham B15 2TT, United Kingdom. Phone: (44) 121 414 8174. Fax: (44) 121 414 3599. E-mail: p.balfe{at}bham.ac.uk

{triangledown} Published ahead of print on 24 October 2007.

Journal of Virology, January 2008, p. 461-470, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.01894-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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