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Journal of Virology, May 2007, p. 4881-4885, Vol. 81, No. 9
0022-538X/07/$08.00+0     doi:10.1128/JVI.02210-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Rab 5 Is Required for the Cellular Entry of Dengue and West Nile Viruses{triangledown}

Manoj N. Krishnan,1 Bindu Sukumaran,1 Utpal Pal,1 Herve Agaisse,2 James L. Murray,3 Thomas W. Hodge,3 and Erol Fikrig1,2*

Section of Rheumatology, Department of Internal Medicine,1 Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut,2 University of Georgia, College of Veterinary Medicine, Department of Infectious Diseases, Athens, Georgia3

Received 8 October 2006/ Accepted 31 January 2007

The mechanisms of cellular entry of dengue and West Nile viruses are not well characterized. We show that both these viruses enter HeLa cells by clathrin-dependent endocytosis and require vacuolar acidic pH. Inhibition of the GTPase Rab 5 or 7, which regulates transport to early or late endosomes, respectively, demonstrated that Rab 5 was essential for survival of both dengue and West Nile virus. These data broaden our understanding of the pathways required for productive dengue and West Nile virus infection and may facilitate new strategies for combating disease.

* Corresponding author. Mailing address: Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, The Anlyan Center for Medical Research and Education, Room S525A, 300 Cedar Street, P.O. Box 208031, New Haven, Connecticut 06520-8031. Phone: (203) 785-2453. Fax: (203) 785-7053. E-mail: erol.fikrig{at}yale.edu

{triangledown} Published ahead of print on 14 February 2007.

Journal of Virology, May 2007, p. 4881-4885, Vol. 81, No. 9
0022-538X/07/$08.00+0     doi:10.1128/JVI.02210-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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