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Journal of Virology, May 2007, p. 4872-4876, Vol. 81, No. 9
0022-538X/07/$08.00+0     doi:10.1128/JVI.02148-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

PrPSc in Salivary Glands of Scrapie-Affected Sheep{triangledown}

Marta Vascellari,1* Romolo Nonno,2 Franco Mutinelli,1 Michela Bigolaro,1 Michele Angelo Di Bari,2 Erica Melchiotti,1 Stefano Marcon,2 Claudia D'Agostino,2 Gabriele Vaccari,2 Michela Conte,2 Luigi De Grossi,3 Francesca Rosone,3 Francesco Giordani,3 and Umberto Agrimi2

Istituto Zooprofilattico Sperimentale delle Venezie, Histopathology Department, Viale dell'Università 10, 35020 Legnaro (PD), Italy,1 Istituto Superiore di Sanità, Department of Food Safety and Animal Health, Viale Regina Elena 299, 00161 Roma, Italy,2 Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Strada Terme, 01100 Viterbo, Italy3

Received 29 September 2006/ Accepted 6 February 2007

The salivary glands of scrapie-affected sheep and healthy controls were investigated for the presence of the pathological prion protein (PrPSc). PrPSc was detected in major (parotid and mandibular) and minor (buccal, labial, and palatine) salivary glands of naturally and experimentally infected sheep. Using Western blotting, the PrPSc concentration in glands was estimated to be 0.02 to 0.005% of that in brain. Immunohistochemistry revealed intracellular depositions of PrPSc in ductal and acinar epithelia and occasional labeling in the lumina of salivary ducts. The presence of PrPSc in salivary glands highlights the possible role of saliva in the horizontal transmission of scrapie.

* Corresponding author. Mailing address: Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro (PD), Italy. Phone: 39 049 8084259. Fax: 39 049 8084258. E-mail: mvascellari{at}izsvenezie.it

{triangledown} Published ahead of print on 14 February 2007.

Journal of Virology, May 2007, p. 4872-4876, Vol. 81, No. 9
0022-538X/07/$08.00+0     doi:10.1128/JVI.02148-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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