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Journal of Virology, May 2007, p. 4604-4614, Vol. 81, No. 9
0022-538X/07/$08.00+0     doi:10.1128/JVI.02138-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Stability of a Receptor-Binding Active Human Immunodeficiency Virus Type 1 Recombinant gp140 Trimer Conferred by Intermonomer Disulfide Bonding of the V3 Loop: Differential Effects of Protein Disulfide Isomerase on CD4 and Coreceptor Binding

J. Billington,¹ T. P. Hickling,^1, G. H. Munro,¹ C. Halai,¹ R. Chung,¹ G. G. Dodson,² and R. S. Daniels^1*

Virology Division,¹ Protein Structure Division, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom²

Received 29 September 2006/ Accepted 8 February 2007

Stable trimeric forms of human immunodeficiency virus recombinant gp140 (rgp140) are important templates for determining the structure of the glycoprotein to assist in our understanding of HIV infection and host immune response. Such information will aid the design of therapeutic drugs and vaccines. Here, we report the production of a highly stable and trimeric rgp140 derived from a HIV type 1 (HIV-1) subtype D isolate that may be suitable for structural studies. The rgp140 is functional in terms of binding to CD4 and three human monoclonal antibodies (17b, b12, and 2G12) that have broad neutralizing activities against a range of HIV-1 isolates from different subtypes. Treatment of rgp140 with protein disulfide isomerase (PDI) severely restricted 17b binding capabilities. The stable nature of the rgp140 was due to the lack of processing at the gp120/41 boundary and the presence of an intermonomer disulfide bond formed by the cysteines of the V3 loop. Further characterization showed the intermonomer disulfide bond to be a target for PDI processing. The relevance of these findings to the roles of the V3 domain and the timing of PDI action during the HIV infection process are discussed.

Published ahead of print on 14 February 2007.

Present address: Microbiology and Infectious Diseases, West Block, Floor A, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom.