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Journal of Virology, April 2007, p. 4378-4380, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02246-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Xenopus Xp54 and Human RCK/p54 Helicases Functionally Replace Yeast Dhh1p in Brome Mosaic Virus RNA Replication{triangledown}

Isabel Alves-Rodrigues,1 Antonio Mas,1,2 and Juana Díez1*

Departamento de Ciencias Experimentales y de la Salud, Universitat Pompeu Fabra, 08003 Barcelona, Spain,1 Centro Regional de Investigaciones Biomédicas-Facultad de Medicina, Universidad de Castilla La Mancha, Albacete, Spain2

Received 13 October 2006/ Accepted 24 January 2007

By using a Brome mosaic virus (BMV)-Saccharomyces cerevisiae system, we previously showed that the cellular Lsm1p-7p/Pat1p/Dhh1p decapping-activator complex functions in BMV RNA translation and replication. As a first approach in investigating whether the corresponding human homologues play a similar role, we expressed human Lsm1p (hLsm1p) and RCK/p54 in yeast. Expression of RCK/p54 but not hLsm1p restored the defect in BMV RNA translation and replication observed in the dhh1{Delta} and lsm1{Delta} strains, respectively. This functional conservation, together with the common replication strategies of positive-stranded RNA viruses, suggests that RCK/p54 may also play a role in the replication of positive-stranded RNA viruses that infect humans.

* Corresponding author. Mailing address: Departamento Ciencias Experimentales y de la Salud, Universitat Pompeu Fabra, Dr. Aiguader 80, 08003 Barcelona, Spain. Phone: 34-93-542-2887. Fax: 34-93-542-2802. E-mail: juana.diez{at}upf.edu

{triangledown} Published ahead of print on 14 February 2007.

Journal of Virology, April 2007, p. 4378-4380, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02246-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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