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Journal of Virology, April 2007, p. 4371-4373, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02672-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Distinct Sites on the Sindbis Virus RNA-Dependent RNA Polymerase for Binding to the Promoters for the Synthesis of Genomic and Subgenomic RNA

Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey—Robert Wood Johnson Medical School, Piscataway, New Jersey 08854,¹ Cancer Institute of New Jersey, New Brunswick, New Jersey 08901²

Received 4 December 2006/ Accepted 29 January 2007

Sindbis virus-infected cells make two positive-strand RNAs, a genomic (G) RNA and a subgenomic (SG) RNA. Here we report the amino acid sequence in nonstructural protein 4 (nsP4), the viral RNA-dependent RNA polymerase, that binds to the promoter for the synthesis of G RNA. In addition, using a cell-free system that makes both G and SG RNA, we show that specific amino acid changes in nsP4 that abolish the synthesis of SG RNA have no effect on the synthesis of G RNA. Our findings indicate that nsP4 has distinct sites for the recognition of the G and SG promoters.

Published ahead of print on 7 February 2007.

This article has been cited by other articles:

• Li, M.-L., Kwan, T.-Y., Simmonds, H. A., Stollar, V. (2008). Synthesis of Genomic and Subgenomic RNA in Mosquito Cells Infected with Two Sindbis Virus nsP4 Mutants: Influence of Intracellular Nucleoside Triphosphate Concentrations. J. Virol. 82: 6880-6888 [Abstract] [Full Text]