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Journal of Virology, April 2007, p. 4367-4370, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02357-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Efficiency of Human Immunodeficiency Virus Type 1 Postentry Infection Processes: Evidence against Disproportionate Numbers of Defective Virions{triangledown}

James A. Thomas, David E. Ott, and Robert J. Gorelick*

AIDS Vaccine Program, Basic Research Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland 21702

Received 27 October 2006/ Accepted 23 January 2007

The vast majority of human immunodeficiency virus type 1 particles are claimed to be noninfectious, but there is disagreement as to whether they are defective or simply lack the opportunity to initiate an infection. We have examined the efficiencies of reverse transcription and integration and find that approximately 1 of every 8 virions that initiate reverse transcription form proviruses, a quantity significantly different from the commonly reported ratio of 1 in 1,000. In addition, results from two different infectivity assays demonstrate that the titers are not equivalent to the number of infectious particles. The apparent predominance of noninfectious particles is due to infrequent occurrences of successful virus-cell interactions.

* Corresponding author. Mailing address: Building 535, 4th Floor, National Cancer Institute, Frederick, MD 21702. Phone: (301) 846-5980. Fax: (301) 846-7119. E-mail: gorelick{at}ncifcrf.gov

{triangledown} Published ahead of print on 31 January 2007.

Journal of Virology, April 2007, p. 4367-4370, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02357-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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