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Journal of Virology, April 2007, p. 4305-4314, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02474-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Transmission and Adaptation of Chronic Wasting Disease to Hamsters and Transgenic Mice: Evidence for Strains{triangledown}

Gregory J. Raymond,1 Lynne D. Raymond,1 Kimberly D. Meade-White,1 Andrew G. Hughson,1 Cynthia Favara,1 Donald Gardner,2 Elizabeth S. Williams,3,{dagger} Michael W. Miller,4 Richard E. Race,1* and Byron Caughey1*

Laboratory of Persistent Viral Diseases,1 Rocky Mountain Veterinary Branch, NIAID, NIH, Rocky Mountain Laboratories, Hamilton, Montana 59840,2 Department of Veterinary Sciences, University of Wyoming, Laramie, Wyoming 82070,3 Colorado Division of Wildlife, Wildlife Research Center, Fort Collins, Colorado 80526-20974

Received 9 November 2006/ Accepted 23 January 2007

In vitro screening using the cell-free prion protein conversion system indicated that certain rodents may be susceptible to chronic wasting disease (CWD). Therefore, CWD isolates from mule deer, white-tailed deer, and elk were inoculated intracerebrally into various rodent species to assess the rodents' susceptibility and to develop new rodent models of CWD. The species inoculated were Syrian golden, Djungarian, Chinese, Siberian, and Armenian hamsters, transgenic mice expressing the Syrian golden hamster prion protein, and RML Swiss and C57BL10 wild-type mice. The transgenic mice and the Syrian golden, Chinese, Siberian, and Armenian hamsters had limited susceptibility to certain of the CWD inocula, as evidenced by incomplete attack rates and long incubation periods. For serial passages of CWD isolates in Syrian golden hamsters, incubation periods rapidly stabilized, with isolates having either short (85 to 89 days) or long (408 to 544 days) mean incubation periods and distinct neuropathological patterns. In contrast, wild-type mouse strains and Djungarian hamsters were not susceptible to CWD. These results show that CWD can be transmitted and adapted to some species of rodents and suggest that the cervid-derived CWD inocula may have contained or diverged into at least two distinct transmissible spongiform encephalopathy strains.

* Corresponding author. Mailing address: Rocky Mountain Labs, 903 S. 4th St., Hamilton, MT 59840. Phone for Byron Caughey: (406) 363-9264. Fax: (406) 363-9286. E-mail: bcaughey{at}nih.gov. Phone for Richard Race: (406) 363-9358. Fax: (406) 363-9286. E-mail: rrace{at}nih.gov

{triangledown} Published ahead of print on 7 February 2007.

{dagger} Deceased.

Journal of Virology, April 2007, p. 4305-4314, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02474-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Perucchini, M., Griffin, K., Miller, M. W., Goldmann, W. (2008). PrP genotypes of free-ranging wapiti (Cervus elaphus nelsoni) with chronic wasting disease. J. Gen. Virol. 89: 1324-1328 [Abstract] [Full Text]  
  • Hamir, A. N., Richt, J. A., Miller, J. M., Kunkle, R. A., Hall, S. M., Nicholson, E. M., O'Rourke, K. I., Greenlee, J. J., Williams, E. S. (2008). Experimental Transmission of Chronic Wasting Disease (CWD) of Elk (Cervus elaphus nelsoni), White-tailed Deer (Odocoileus virginianus), and Mule Deer (Odocoileus hemionus hemionus) to White-tailed Deer by Intracerebral Route. Vet Pathol 45: 297-306 [Abstract] [Full Text]  


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