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Journal of Virology, April 2007, p. 3949-3968, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02333-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Kaposi's Sarcoma-Associated Herpesvirus Induces Sustained NF-{kappa}B Activation during De Novo Infection of Primary Human Dermal Microvascular Endothelial Cells That Is Essential for Viral Gene Expression{triangledown}

Sathish Sadagopan, Neelam Sharma-Walia, Mohanan Valiya Veettil, Hari Raghu, Ramu Sivakumar, Virginie Bottero, and Bala Chandran*

Department of Microbiology and Immunology, H. M. Bligh Cancer Research Laboratories, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois

Received 24 October 2006/ Accepted 24 January 2007

In vitro Kaposi's sarcoma-associated herpesvirus (KSHV) infection of primary human dermal microvascular endothelial (HMVEC-d) cells and human foreskin fibroblast (HFF) cells is characterized by the induction of preexisting host signal cascades, sustained expression of latency-associated genes, transient expression of a limited number of lytic genes, and induction of several cytokines, growth factors, and angiogenic factors. Since NF-{kappa}B is a key molecule involved in the regulation of several of these factors, here, we examined NF-{kappa}B induction during de novo infection of HMVEC-d and HFF cells. Activation of NF-{kappa}B was observed as early as 5 to 15 min postinfection by KSHV, and translocation of p65-NF-{kappa}B into nuclei was detected by immunofluorescence assay, electrophoretic mobility shift assay, and p65 enzyme-linked immunosorbent assay. I{kappa}B phosphorylation inhibitor (Bay11-7082) reduced this activation significantly. A sustained moderate level of NF-{kappa}B induction was seen during the observed 72 h of in vitro KSHV latency. In contrast, high levels of ERK1/2 activation at earlier time points and a moderate level of activation at later times were observed. p38 mitogen-activated protein kinase was activated only at later time points, and AKT was activated in a cyclic manner. Studies with UV-inactivated KSHV suggested a role for virus entry stages in NF-{kappa}B induction and a requirement for KSHV viral gene expression in sustained induction. Inhibition of NF-{kappa}B did not affect target cell entry by KSHV but significantly reduced the expression of viral latent open reading frame 73 and lytic genes. KSHV infection induced the activation of several host transcription factors, including AP-1 family members, as well as several cytokines, growth factors, and angiogenic factors, which were significantly affected by NF-{kappa}B inhibition. These results suggest that during de novo infection, KSHV induces sustained levels of NF-{kappa}B to regulate viral and host cell genes and thus possibly regulates the establishment of latent infection.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL 60064. Phone: (847) 578-8822. Fax: (847) 578-3349. E-mail: bala.chandran{at}rosalindfranklin.edu

{triangledown} Published ahead of print on 7 February 2007.

Journal of Virology, April 2007, p. 3949-3968, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.02333-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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