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Journal of Virology, April 2007, p. 3757-3768, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.01544-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Impact of V2 Mutations on Escape from a Potent Neutralizing Anti-V3 Monoclonal Antibody during In Vitro Selection of a Primary Human Immunodeficiency Virus Type 1 Isolate{triangledown}

Junji Shibata,1 Kazuhisa Yoshimura,1 Akiko Honda,1 Atsushi Koito,1 Toshio Murakami,2 and Shuzo Matsushita1*

Division of Clinical Retrovirology and Infectious Diseases, Center for AIDS Research, Kumamoto University, Kumamoto 860-0811,1 The Chemo-Sero-Therapeutic Research Institute, Kyokushi, Kikuchi, Kumamoto 869-1298, Japan2

Received 19 July 2006/ Accepted 16 January 2007

KD-247, a humanized monoclonal antibody to an epitope of gp120-V3 tip, has potent cross-neutralizing activity against subtype B primary human immunodeficiency virus type 1 (HIV-1) isolates. To assess how KD-247 escape mutants can be generated, we induced escape variants by exposing bulked primary R5 virus, MOKW, to increasing concentrations of KD-247 in vitro. In the presence of relatively low concentrations of KD-247, viruses with two amino acid mutations (R166K/D167N) in V2 expanded, and under high KD-247 pressure, a V3 tip substitution (P313L) emerged in addition to the V2 mutations. However, a virus with a V2 175P mutation dominated during passaging in the absence of KD-247. Using domain swapping analysis, we demonstrated that the V2 mutations and the P313L mutation in V3 contribute to partial and complete resistance phenotypes against KD-247, respectively. To identify the V2 mutation responsible for the resistance to KD-247, we constructed pseudoviruses with single or double amino acid mutations in V2 and measured their sensitivity to neutralization. Interestingly, the neutralization phenotypes were switched, so that amino acid residue 175 (Pro or Leu) located in the center of V2 was exchanged, indicating that the amino acid at position 175 has a crucial role, dramatically changing the Env oligomeric state on the membrane surface and affecting the neutralization phenotype against not only anti-V3 antibody but also recombinant soluble CD4. These data suggested that HIV-1 can escape from anti-V3 antibody attack by changing the conformation of the functional envelope oligomer by acquiring mutations in the V2 region in environments with relatively low antibody concentrations.

* Corresponding author. Mailing address: Division of Clinical Retrovirology and Infectious Diseases, Center for AIDS Research, Kumamoto University, Kumamoto 860-0811, Japan. Phone: 81 96 373 6536. Fax: 81 96 373 6537. E-mail: shuzo{at}kaiju.medic.kumamoto-u.ac.jp

{triangledown} Published ahead of print on 24 January 2007.

Journal of Virology, April 2007, p. 3757-3768, Vol. 81, No. 8
0022-538X/07/$08.00+0     doi:10.1128/JVI.01544-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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