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Journal of Virology, April 2007, p. 3652-3656, Vol. 81, No. 7
0022-538X/07/$08.00+0     doi:10.1128/JVI.02105-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

New Way of Regulating Alternative Splicing in Retroviruses: the Promoter Makes a Difference{triangledown}

Jens Bohne,* Axel Schambach, and Daniela Zychlinski

Experimental Hematology, Hannover Medical School, D-30625 Hanover, Germany

Received 26 September 2006/ Accepted 5 January 2007

Alternative splicing has been recognized as a major mechanism for creating proteomic diversity from a limited number of genes. However, not all determinants regulating this process have been characterized. Using subviral human immunodeficiency virus (HIV) env constructs we observed an enhanced splicing of the RNA when expression was under control of the cytomegalovirus (CMV) promoter instead of the HIV long terminal repeat (LTR). We extended these observations to LTR- or CMV-driven murine leukemia proviruses, suggesting that retroviral LTRs are adapted to inefficient alternative splicing at most sites in order to maintain balanced gene expression.

* Corresponding author. Mailing address: Experimental Hematology, Hannover Medical School, Carl-Neuberg-Str. 1, OE 6960, D-30625 Hanover, Germany. Phone: 49 511 532-6066. Fax: 49 511 532-6987. E-mail: bohne.jens{at}mh-hannover.de.

{triangledown} Published ahead of print on 17 January 2007.

Journal of Virology, April 2007, p. 3652-3656, Vol. 81, No. 7
0022-538X/07/$08.00+0     doi:10.1128/JVI.02105-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zychlinski, D., Erkelenz, S., Melhorn, V., Baum, C., Schaal, H., Bohne, J. (2009). Limited complementarity between U1 snRNA and a retroviral 5' splice site permits its attenuation via RNA secondary structure. Nucleic Acids Res 0: gkp694v1-gkp694 [Abstract] [Full Text]  
  • Jackson, D., Lamb, R. A. (2008). The influenza A virus spliced messenger RNA M mRNA3 is not required for viral replication in tissue culture. J. Gen. Virol. 89: 3097-3101 [Abstract] [Full Text]  


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