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Journal of Virology, April 2007, p. 3627-3631, Vol. 81, No. 7
0022-538X/07/$08.00+0     doi:10.1128/JVI.02786-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Influence of Coagulation Factor Zymogens on the Infectivity of Adenoviruses Pseudotyped with Fibers from Subgroup D{triangledown}

Alan L. Parker,1 John H. McVey,2 Jessica H. Doctor,1 Oscar Lopez-Franco,1 Simon N. Waddington,3 Menzo J. E. Havenga,4 Stuart A. Nicklin,1 and Andrew H. Baker1*

British Heart Foundation Glasgow Cardiovascular Research Centre, Division of Cardiovascular and Medical Sciences, University of Glasgow, 126 University Place, Glasgow G12 8TA, United Kingdom,1 Haemostasis and Thrombosis, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, United Kingdom,2 Department of Haematology, Haemophilia Centre and Haemostasis Unit, Royal Free and University College Medical School, London NW3 2PF, United Kingdom,3 Crucell, P.O. Box 2048, 2301 CA Leiden, The Netherlands4

Received 18 December 2006/ Accepted 16 January 2007

Recent evidence supports a role for vitamin K-dependent coagulation zymogens in adenovirus serotype 5 (Ad5, subgroup C) infection of hepatocytes. Here, we assessed the effect of virus-zymogen interaction on cellular transduction using a panel of fiber (f)-pseudotyped viruses derived from subgroup D (f47, f33, f24, f45, f17, f30). Each virus directly bound factor X (FX) as determined by surface plasmon resonance, resulting in enhanced cell surface binding. Infection of HepG2 cells was promoted by FX but not by FVII or FIX, while transduction of CHO cells was blocked in heparan sulfate proteoglycan-deficient cells. This suggests a broad role for FX in adenovirus infectivity.

* Corresponding author. Mailing address: British Heart Foundation Glasgow Cardiovascular Research Centre, Division of Cardiovascular and Medical Sciences, University of Glasgow, 126 University Place, Glasgow G12 8TA, United Kingdom. Phone: 44 141 330 1977. Fax: 44 141 330 6997. E-mail: ab11f{at}clinmed.gla.ac.uk.

{triangledown} Published ahead of print on 24 January 2007.

Journal of Virology, April 2007, p. 3627-3631, Vol. 81, No. 7
0022-538X/07/$08.00+0     doi:10.1128/JVI.02786-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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