This Article Full Text Full Text (PDF) Other Versions of this Article: JVI.02569-06v1 81/7/3574    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vatakis, D. N. Articles by Zack, J. A. Search for Related Content PubMed PubMed Citation Articles by Vatakis, D. N. Articles by Zack, J. A.

Immediate Activation Fails To Rescue Efficient Human Immunodeficiency Virus Replication in Quiescent CD4⁺ T Cells

Division of Hematology and Oncology, Department of Medicine,¹ Department of Molecular and Medical Pharmacology,² Department of Microbiology, Immunology and Molecular Genetics,³ UCLA AIDS Institute, David Geffen School of Medicine at UCLA, Los Angeles, California 90095⁴

Received 21 November 2006/ Accepted 4 January 2007

Unlike activated T cells, quiescent CD4⁺ T cells have shown resistance to human immunodeficiency virus (HIV) infection due to a block in the early events of the viral life cycle. To further investigate the nature of this block, we infected quiescent CD4⁺ T cells with HIV-1_NL4-3 and immediately stimulated them. Compared to activated (prestimulated) cells, these poststimulated cells showed slightly decreased viral entry and delays in the completion of reverse transcription. However, the relative efficiency of integration was similar to that of prestimulated cells. Together, this resulted in decreased expression of tat/rev mRNA and synthesis of viral protein. Furthermore, based on cell cycle staining and BrdU incorporation, poststimulated cells expressing viral protein failed to initiate a second round of their cell cycle, independently of Vpr-mediated arrest. Together, these data demonstrate that the early stages of the HIV life cycle are inefficient in these poststimulated cells and that efficient replication cannot be induced by subsequent activation.

Published ahead of print on 17 January 2007.

This article has been cited by other articles:

• Plesa, G., Dai, J., Baytop, C., Riley, J. L., June, C. H., O'Doherty, U. (2007). Addition of Deoxynucleosides Enhances Human Immunodeficiency Virus Type 1 Integration and 2LTR Formation in Resting CD4+ T Cells. J. Virol. 81: 13938-13942 [Abstract] [Full Text]  
• Burke, B., Brown, H. J., Marsden, M. D., Bristol, G., Vatakis, D. N., Zack, J. A. (2007). Primary Cell Model for Activation-Inducible Human Immunodeficiency Virus. J. Virol. 81: 7424-7434 [Abstract] [Full Text]