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Journal of Virology, March 2007, p. 3042-3048, Vol. 81, No. 6
0022-538X/07/$08.00+0     doi:10.1128/JVI.01564-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Cytomegalovirus Primary Envelopment Occurs at Large Infoldings of the Inner Nuclear Membrane{triangledown}

Christopher Buser,1,2 Paul Walther,1 Thomas Mertens,2* and Detlef Michel2

Zentrale Einrichtung Elektronenmikroskopie, Universität Ulm,1 Institut für Virologie, Universitätsklinikum Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany2

Received 21 July 2006/ Accepted 14 December 2006

We have investigated the morphogenesis of human and murine cytomegalovirus by transmission electron microscopy after high-pressure freezing, freeze substitution, and plastic embedding. We observed large tubular infoldings of the inner nuclear membrane that were free of lamina and active in primary envelopment and subsequent transport of capsids to the nuclear periphery. Semiquantitative determinations of the enlarged inner nuclear membrane area and the location of the primary envelopment of nucleocapsids demonstrated that this structure represents a virus-induced specialized membrane domain at which the particles are preferentially enveloped. This is a previously undescribed structural element relevant in cytomegalovirus morphogenesis.

* Corresponding author. Mailing address: Universitätsklinikum Ulm, Institut für Virologie, Albert Einstein Allee 11, 89081 Ulm, Germany. Phone: 49 731 500 65100. Fax: 49 731 500 65102. E-mail: thomas.mertens{at}uniklinik-ulm.de.

{triangledown} Published ahead of print on 27 December 2006.

Journal of Virology, March 2007, p. 3042-3048, Vol. 81, No. 6
0022-538X/07/$08.00+0     doi:10.1128/JVI.01564-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Milbradt, J., Auerochs, S., Sticht, H., Marschall, M. (2009). Cytomegaloviral proteins that associate with the nuclear lamina: components of a postulated nuclear egress complex. J. Gen. Virol. 90: 579-590 [Abstract] [Full Text]  
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