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Journal of Virology, March 2007, p. 2605-2613, Vol. 81, No. 6
0022-538X/07/$08.00+0     doi:10.1128/JVI.02313-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Upstream AP1- and CREB-Binding Sites Confer High Basal Activity on the Adeno-Associated Virus Type 5 Capsid Gene Promoter{triangledown}

Chaoyang Ye and David J. Pintel*

Department of Molecular Microbiology and Immunology, University of Missouri—Columbia, School of Medicine, Life Sciences Center, Columbia, Missouri 65211

Received 20 October 2006/ Accepted 21 December 2006

In contrast to the prototype adeno-associated virus type 2 (AAV2), the capsid gene P41 promoter of AAV5, within viral constructs that lack inverted terminal repeat sequences, displays a high basal level of expression in 293 cells in the absence of coinfecting adenovirus. Here we demonstrate that this was due to differences in the relative strengths of the core promoter elements and to the presence of active binding sites for the transcription factors CREB and AP1 within the upstream region of P41 that are absent from the AAV2 capsid gene promoter P40. These differences also governed the relative basal activity of the AAV capsid gene promoters within near-full-length viral genomes.

* Corresponding author. Mailing address: University of Missouri Medical School, 471f Life Sciences Building, 1201 E. Rollins Rd., Columbia, MO 65211-7310. Phone: (573) 882-3920. Fax: (573) 884-9676. E-mail: pinteld{at}missouri.edu.

{triangledown} Published ahead of print on 3 January 2007.

Journal of Virology, March 2007, p. 2605-2613, Vol. 81, No. 6
0022-538X/07/$08.00+0     doi:10.1128/JVI.02313-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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