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Journal of Virology, March 2007, p. 2524-2530, Vol. 81, No. 5
0022-538X/07/$08.00+0     doi:10.1128/JVI.01931-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Histone Modification Pattern of the T-Cellular Herpesvirus Saimiri Genome in Latency{triangledown}

Barbara Alberter and Armin Ensser*

Institut für Klinische und Molekulare Virologie, Friedrich-Alexander Universität Erlangen-Nürnberg, D-91054 Erlangen, Germany

Received 5 September 2006/ Accepted 29 November 2006

Herpesvirus saimiri (HVS) subgroup C strains are able to growth transform human T lymphocytes in vitro. The stably persisting and nonintegrating HVS episome represents an optimal prerequisite for the investigation of the epigenetic state of latent herpesvirus genomes in vitro. Quantitative chromatin immunoprecipitation experiments using seven different histone acetylation- or methylation-specific antibodies revealed repressive marks at four lytic gene promoters and a variable pattern at the weakly transcribed LANA/orf73 promoter. The constitutive stpC/tip promoter regulating the viral oncoproteins and, more interestingly, the noncoding repetitive H-DNA elements flanking the coding region, showed a permissive chromatin structure. This study provides an appropriate model for the analysis of epigenetic herpesvirus genome modifications and their dynamics in T cells.

* Corresponding author. Mailing address: Institut für Klinische und Molekulare Virologie, Friedrich-Alexander Universität Erlangen-Nürnberg, Schlossgarten 4, D-91054 Erlangen, Germany. Phone: 49-9131-85-22104. Fax: 49-9131-85-26493. E-mail: armin.ensser{at}viro.med.uni-erlangen.de.

{triangledown} Published ahead of print on 6 December 2006.

Journal of Virology, March 2007, p. 2524-2530, Vol. 81, No. 5
0022-538X/07/$08.00+0     doi:10.1128/JVI.01931-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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