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Journal of Virology, March 2007, p. 2519-2523, Vol. 81, No. 5
0022-538X/07/$08.00+0     doi:10.1128/JVI.01661-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Dendritic Cell-Mediated trans-Enhancement of Human Immunodeficiency Virus Type 1 Infectivity Is Independent of DC-SIGN{triangledown}

Cesar Boggiano,1,{dagger} Nicolas Manel,1,{dagger} and Dan R. Littman1,2*

Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016,1 The Howard Hughes Medical Institute, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 100162

Received 2 August 2006/ Accepted 8 December 2006

Dendritic cells (DCs) enhance human immunodeficiency virus type 1 (HIV-1) infection of CD4+ T lymphocytes in trans. The C-type lectin DC-SIGN, expressed on DCs, binds to the HIV-1 envelope glycoprotein gp120 and confers upon some cell lines the capacity to enhance trans-infection. Using a short hairpin RNA approach, we demonstrate that DC-SIGN is not required for efficient trans-enhancement by DCs. In addition, the DC-SIGN ligand mannan and an anti-DC-SIGN antibody did not inhibit DC-mediated enhancement. HIV-1 particles were internalized and were protected from protease treatment following binding to DCs, but not from binding to DC-SIGN-expressing Raji cells. Thus, DC-SIGN is not required for DC-mediated trans-enhancement of HIV infectivity.

* Corresponding author. Mailing address: Skirball Institute of Biomolecular Medicine, 540 First Avenue, New York, NY 10016. Phone: (212) 263-7520. Fax: (212) 263-1498. E-mail: littman{at}saturn.mail.nyu.edu.

{triangledown} Published ahead of print on 20 December 2006.

{dagger} These authors contributed equally to this work.

Journal of Virology, March 2007, p. 2519-2523, Vol. 81, No. 5
0022-538X/07/$08.00+0     doi:10.1128/JVI.01661-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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