This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Peng, Y. Articles by Yilma, T. D. Search for Related Content PubMed PubMed Citation Articles by Peng, Y. Articles by Yilma, T. D.

 Previous Article  |  Next Article 

Journal of Virology, March 2007, p. 2187-2195, Vol. 81, No. 5
0022-538X/07/$08.00+0     doi:10.1128/JVI.01879-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Pseudotyped Single-Cycle Simian Immunodeficiency Viruses Expressing Gamma Interferon Augment T-Cell Priming Responses In Vitro

Yue Peng,¹ Fan-ching Lin,¹ Paulo H. Verardi,¹ Leslie A. Jones,¹ Michael B. McChesney,³ and Tilahun D. Yilma^1,2*

International Laboratory of Molecular Biology for Tropical Disease Agents, School of Veterinary Medicine,¹ Department of Medical Microbiology and Immunology, School of Medicine,² California National Primate Research Center and Department of Pathology, School of Medicine, University of California, Davis, California 95616³

Received 30 August 2006/ Accepted 3 December 2006

To increase the safety and efficacy of human immunodeficiency virus vaccines, several groups have conducted studies using the macaque model with single-cycle replicating simian immunodeficiency viruses (SIVs). However, these constructs had poor or diminished efficacy compared to live attenuated vaccines. We previously showed that immunization of macaques with live attenuated SIV with a deletion in the nef gene and expressing gamma interferon (IFN-) results in significantly enhanced safety and efficacy. To further enhance safety, we constructed and characterized single-cycle SIVs, pseudotyped with the glycoprotein of vesicular stomatitis virus, expressing different levels of macaque IFN-. Expression of IFN- did not alter the infectivity or antigenicity of pseudotyped SIV. The transduction of dendritic cells (DCs) by IFN--expressing particles resulted in the up-regulation of costimulatory and major histocompatibility complex molecules. Furthermore, T cells primed with DCs transduced by SIV particles expressing high levels of IFN- and then stimulated with SIV induced significantly higher numbers of spot-forming cells in an enzyme-linked immunospot assay than did T cells primed with DCs transduced with SIV particles lacking the cytokine. In conclusion, we demonstrated that the transduction of DCs in vitro with pseudotyped single-cycle SIVs expressing IFN- increased DC activation and augmented T-cell priming activity.

---

* Corresponding author. Mailing address: International Laboratory of Molecular Biology for Tropical Disease Agents, University of California, Davis, CA 95616. Phone: (530) 752-8306. Fax: (530) 752-1354. E-mail: tdyilma{at}ucdavis.edu.

Published ahead of print on 13 December 2006.

---

Journal of Virology, March 2007, p. 2187-2195, Vol. 81, No. 5
0022-538X/07/$08.00+0     doi:10.1128/JVI.01879-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Peng, Y., Lin, F.-c., Verardi, P. H., Jones, L. A., Yilma, T. D. (2009). Lower Levels of Gamma Interferon Expressed by a Pseudotyped Single-Cycle Simian Immunodeficiency Virus Enhance Immunogenicity in Rats. J. Virol. 83: 1592-1601 [Abstract] [Full Text]  
• Lin, F.-c., Peng, Y., Jones, L. A., Verardi, P. H., Yilma, T. D. (2009). Incorporation of CD40 Ligand into the Envelope of Pseudotyped Single-Cycle Simian Immunodeficiency Viruses Enhances Immunogenicity. J. Virol. 83: 1216-1227 [Abstract] [Full Text]