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Journal of Virology, February 2007, p. 2074-2077, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.01781-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evidence for the Existence of the Loop E Motif of Potato Spindle Tuber Viroid In Vivo

Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center,¹ The OSU RNA Group, Ohio State University, Columbus, Ohio 43210²

Received 16 August 2006/ Accepted 16 November 2006

RNA motifs comprising nucleotides that interact through non-Watson-Crick base pairing play critical roles in RNA functions, often by serving as the sites for RNA-RNA, RNA-protein, or RNA small ligand interactions. The structures of viral and viroid RNA motifs are studied commonly by in vitro, computational, and mutagenesis approaches. Demonstration of the in vivo existence of a motif will help establish its biological significance and promote mechanistic studies on its functions. By using UV cross-linking and primer extension, we have obtained direct evidence for the in vivo existence of the loop E motif of Potato spindle tuber viroid. We present our findings and discuss their biological implications.

Published ahead of print on 29 November 2006.

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