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Journal of Virology, February 2007, p. 2074-2077, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.01781-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evidence for the Existence of the Loop E Motif of Potato Spindle Tuber Viroid In Vivo{triangledown}

Ying Wang,1 Xuehua Zhong,1 Asuka Itaya,1 and Biao Ding1,2*

Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center,1 The OSU RNA Group, Ohio State University, Columbus, Ohio 432102

Received 16 August 2006/ Accepted 16 November 2006

RNA motifs comprising nucleotides that interact through non-Watson-Crick base pairing play critical roles in RNA functions, often by serving as the sites for RNA-RNA, RNA-protein, or RNA small ligand interactions. The structures of viral and viroid RNA motifs are studied commonly by in vitro, computational, and mutagenesis approaches. Demonstration of the in vivo existence of a motif will help establish its biological significance and promote mechanistic studies on its functions. By using UV cross-linking and primer extension, we have obtained direct evidence for the in vivo existence of the loop E motif of Potato spindle tuber viroid. We present our findings and discuss their biological implications.

* Corresponding author. Mailing address: Department of Plant Cellular and Molecular Biology, Ohio State University, 207 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210. Phone: (614) 247-6077. Fax: (614) 292-5379. E-mail: ding.35{at}osu.edu.

{triangledown} Published ahead of print on 29 November 2006.

Journal of Virology, February 2007, p. 2074-2077, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.01781-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhong, X., Archual, A. J., Amin, A. A., Ding, B. (2008). A Genomic Map of Viroid RNA Motifs Critical for Replication and Systemic Trafficking. Plant Cell 20: 35-47 [Abstract] [Full Text]  
  • Owens, R. A., Baumstark, T. (2007). Structural differences within the loop E motif imply alternative mechanisms of viroid processing. RNA 13: 824-834 [Abstract] [Full Text]  


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