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Journal of Virology, February 2007, p. 1736-1745, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.01638-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Mitotic Kinesin-Like Protein 2 Binds and Colocalizes with Papillomavirus E2 during Mitosis{triangledown}

Ting Yu, Yu-Cai Peng,{dagger} and Elliot J. Androphy*

Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605

Received 31 July 2006/ Accepted 20 November 2006

MKlp2 is a kinesin-like motor protein of the central mitotic spindle required for completion of cytokinesis. Papillomavirus E2 is a sequence specific DNA binding protein that regulates viral transcription and replication and is responsible for partitioning viral episomes into daughter cells during cell division. We demonstrate that MKlp2 specifically associates with the E2 protein during mitosis. Using chromatin immunoprecipitation, we show viral genomes are in complex with MKlp2 only within this stage of cell cycle. By immunofluorescence, a subpopulation of papillomavirus E2 colocalizes with MKlp2 in the midbody/midplate during late mitosis. We conclude that during specific stages of mitosis, the papillomavirus E2 protein binds to MKlp2, and infer that association with this motor protein ensures viral genome partitioning during cytokinesis.

* Corresponding author. Mailing address: UMass Medical School/LRB 328, 364 Plantation St., Worcester, MA 01605. Phone: (508) 856-6605. Fax: (508) 856-6797. E-mail: elliot.androphy{at}umassmed.edu.

{triangledown} Published ahead of print on 29 November 2006.

{dagger} Present address: Cetek Inc., Marlborough, MA 01752.

Journal of Virology, February 2007, p. 1736-1745, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.01638-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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