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Journal of Virology, February 2007, p. 1524-1527, Vol. 81, No. 3
0022-538X/07/$08.00+0     doi:10.1128/JVI.01379-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Microglial Cell Line Established from Prion Protein-Overexpressing Mice Is Susceptible to Various Murine Prion Strains{triangledown}

Yoshifumi Iwamaru,1 Takato Takenouchi,2 Kazumasa Ogihara,2 Megumi Hoshino,1 Masuhiro Takata,1 Morikazu Imamura,1 Yuichi Tagawa,1 Hiroko Hayashi-Kato,1 Yuko Ushiki-Kaku,3 Yoshihisa Shimizu,1 Hiroyuki Okada,1 Morikazu Shinagawa,1 Hiroshi Kitani,2* and Takashi Yokoyama1

Research Center for Prion Diseases, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki 305-8602,1 Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634,2 Nippi Research Institute of Biomatrix, Senjyu-Midroricho 1-1-1, Adachi, Tokyo 120-8601, Japan3

Received 30 June 2006/ Accepted 14 November 2006

Several lines of evidence suggest that microglia have important roles in the pathogenesis of prion diseases. Here, we establish a novel microglial cell line (MG20) from neonatal tga20 mice that overexpress murine prion protein. After exposure to Chandler scrapie, we observed the replication and accumulation of disease-associated forms of the prion protein in MG20 cells up to the 15th passage. Furthermore, MG20 cells were susceptible to ME7, Obihiro scrapie, and bovine spongiform encephalopathy agents. Thus, MG20 cell lines persistently infected with various murine prion strains provide a useful model for the study of the pathogenesis of prion diseases.

* Corresponding author. Mailing address: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, Japan. Phone and fax: 81-29-838-6043. E-mail: kitani{at}affrc.go.jp.

{triangledown} Published ahead of print on 22 November 2006.

Journal of Virology, February 2007, p. 1524-1527, Vol. 81, No. 3
0022-538X/07/$08.00+0     doi:10.1128/JVI.01379-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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