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Not All Cytokine-Producing CD8⁺ T Cells Suppress Simian Immunodeficiency Virus Replication

Wisconsin National Primate Research Center,¹ Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, Madison, Wisconsin 53715-1299,² Department of Biostatistics, University of Alabama at Birmingham, Birmingham, Alabama 35294³

Received 16 August 2006/ Accepted 9 November 2006

Current assays of CD8⁺ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here we show that CD8⁺ T-cell clones recognizing the same epitope vary enormously in the ability to suppress simian immunodeficiency virus SIVmac239 replication in an in vitro suppression assay. However, all Nef_165-173IW9- and Vif_66-73HW8-specific clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce gamma interferon, tumor necrosis factor alpha, or interleukin-2.

Published ahead of print on 29 November 2006.

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