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Addition of Deoxynucleosides Enhances Human Immunodeficiency Virus Type 1 Integration and 2LTR Formation in Resting CD4⁺ T Cells

Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Received 9 August 2007/ Accepted 27 September 2007

Resting CD4⁺ T cells restrict human immunodeficiency virus (HIV) infection at a step prior to integration. Despite this restriction, we showed previously that HIV integration occurs in resting CD4⁺ T cells in vitro, albeit at lower levels than in activated CD4⁺ T cells. Here we show that addition of deoxynucleosides enhanced integration and 2LTR formation in resting CD4⁺ T cells but that the kinetics were still significantly delayed compared to those of activated T cells. Thus, deoxynucleoside addition partially overcomes the restriction to HIV infection in resting CD4⁺ T cells.

Published ahead of print on 10 October 2007.