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Journal of Virology, December 2007, p. 13938-13942, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.01745-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Addition of Deoxynucleosides Enhances Human Immunodeficiency Virus Type 1 Integration and 2LTR Formation in Resting CD4+ T Cells{triangledown}

Gabriela Plesa, Jihong Dai, Cliff Baytop, James L. Riley, Carl H. June, and Una O'Doherty*

Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Received 9 August 2007/ Accepted 27 September 2007

Resting CD4+ T cells restrict human immunodeficiency virus (HIV) infection at a step prior to integration. Despite this restriction, we showed previously that HIV integration occurs in resting CD4+ T cells in vitro, albeit at lower levels than in activated CD4+ T cells. Here we show that addition of deoxynucleosides enhanced integration and 2LTR formation in resting CD4+ T cells but that the kinetics were still significantly delayed compared to those of activated T cells. Thus, deoxynucleoside addition partially overcomes the restriction to HIV infection in resting CD4+ T cells.

* Corresponding author. Mailing address: 3620 Hamilton Walk, 265 JMB, Philadelphia, PA 19104. Phone: (215) 573-7273. Fax: (215) 573-2348. E-mail: unao{at}mail.med.upenn.edu

{triangledown} Published ahead of print on 10 October 2007.

Journal of Virology, December 2007, p. 13938-13942, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.01745-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vatakis, D. N., Kim, S., Kim, N., Chow, S. A., Zack, J. A. (2009). Human Immunodeficiency Virus Integration Efficiency and Site Selection in Quiescent CD4+ T Cells. J. Virol. 83: 6222-6233 [Abstract] [Full Text]  
  • Dai, J., Agosto, L. M., Baytop, C., Yu, J. J., Pace, M. J., Liszewski, M. K., O'Doherty, U. (2009). Human Immunodeficiency Virus Integrates Directly into Naive Resting CD4+ T Cells but Enters Naive Cells Less Efficiently than Memory Cells. J. Virol. 83: 4528-4537 [Abstract] [Full Text]  
  • Vatakis, D. N., Nixon, C. C., Bristol, G., Zack, J. A. (2009). Differentially Stimulated CD4+ T Cells Display Altered Human Immunodeficiency Virus Infection Kinetics: Implications for the Efficacy of Antiviral Agents. J. Virol. 83: 3374-3378 [Abstract] [Full Text]  


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