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Journal of Virology, December 2007, p. 13927-13931, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.00936-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Protective and Broadly Cross-Neutralizing Epitope of Human Papillomavirus L2{triangledown}

Ratish Gambhira,1 Balasubramanyam Karanam,1 Subhashini Jagu,1 Jeffrey N. Roberts,4 Christopher B. Buck,4 Ioannis Bossis,1 Hannah Alphs,1 Timothy Culp,5 Neil D. Christensen,5 and Richard B. S. Roden1,2,3*

Departments of Pathology,1 Oncology,2 Gynecology & Obstetrics, The Johns Hopkins University, Baltimore, Maryland 21231,3 Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20892-4263,4 Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania5

Received 1 May 2007/ Accepted 28 September 2007

We generated a monoclonal antibody, RG-1, that binds to highly conserved L2 residues 17 to 36 and neutralizes human papillomavirus 16 (HPV16) and HPV18. Passive immunotherapy with RG-1 was protective in mice. Antiserum to the HPV16 L2 peptide comprising residues 17 to 36 (peptide 17-36) neutralized pseudoviruses HPV5, HPV6, HPV16, HPV 18, HPV31, HPV 45, HPV 52, HPV 58, bovine papillomavirus 1, and HPV11 native virions. Depletion of HPV16 L2 peptide 17-36-reactive antibodies from cross-neutralizing rabbit and human L2-specific sera abolished cross-neutralization and drastically reduced neutralization of the cognate type. This cross-neutralization of diverse HPVs associated with cervical cancer, genital warts, and epidermodysplasia verruciformis suggests the possibility of a broadly protective, peptide-based vaccine.

* Corresponding author. Mailing address: Room 308, Cancer Research Building 2, Department of Pathology, Johns Hopkins School of Medicine, 1550 Orleans St., Baltimore, MD 21231. Phone: (410) 502-5161. Fax: (443) 287-4295. E-mail: roden{at}jhmi.edu

{triangledown} Published ahead of print on 10 October 2007.

Journal of Virology, December 2007, p. 13927-13931, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.00936-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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