This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ariumi, Y.
Right arrow Articles by Kato, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ariumi, Y.
Right arrow Articles by Kato, N.

 Previous Article  |  Next Article 

Journal of Virology, December 2007, p. 13922-13926, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.01517-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

DDX3 DEAD-Box RNA Helicase Is Required for Hepatitis C Virus RNA Replication{triangledown}

Yasuo Ariumi,1 Misao Kuroki,1 Ken-ichi Abe,1 Hiromichi Dansako,1 Masanori Ikeda,1 Takaji Wakita,2 and Nobuyuki Kato1*

Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Okayama 700-8558,1 Department of Virology II, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan2

Received 11 July 2007/ Accepted 5 September 2007

DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.

* Corresponding author. Mailing address: Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Okayama 700-8558, Japan. Phone: 81 86 235 7386. Fax: 81 86 235 7392. E-mail: nkato{at}md.okayama-u.ac.jp

{triangledown} Published ahead of print on 12 September 2007.

Journal of Virology, December 2007, p. 13922-13926, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.01517-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wang, H., Kim, S., Ryu, W.-S. (2009). DDX3 DEAD-Box RNA Helicase Inhibits Hepatitis B Virus Reverse Transcription by Incorporation into Nucleocapsids. J. Virol. 83: 5815-5824 [Abstract] [Full Text]  
  • Kuroki, M., Ariumi, Y., Ikeda, M., Dansako, H., Wakita, T., Kato, N. (2009). Arsenic Trioxide Inhibits Hepatitis C Virus RNA Replication through Modulation of the Glutathione Redox System and Oxidative Stress. J. Virol. 83: 2338-2348 [Abstract] [Full Text]  
  • Bates, J. S., Petry, D. B., Eudy, J., Bough, L., Johnson, R. K. (2008). Differential expression in lung and bronchial lymph node of pigs with high and low responses to infection with porcine reproductive and respiratory syndrome virus. J ANIM SCI 86: 3279-3289 [Abstract] [Full Text]  
  • Ariumi, Y., Kuroki, M., Dansako, H., Abe, K.-I., Ikeda, M., Wakita, T., Kato, N. (2008). The DNA Damage Sensors Ataxia-Telangiectasia Mutated Kinase and Checkpoint Kinase 2 Are Required for Hepatitis C Virus RNA Replication. J. Virol. 82: 9639-9646 [Abstract] [Full Text]  
  • Lee, C.-S., Dias, A. P., Jedrychowski, M., Patel, A. H., Hsu, J. L., Reed, R. (2008). Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. Nucleic Acids Res 36: 4708-4718 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»