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Journal of Virology, December 2007, p. 13835-13844, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.00914-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

De Novo Synthesis of N and P Proteins as a Key Step in Sendai Virus Gene Expression{triangledown}

Marian A. Wiegand,1 Sascha Bossow,1 Sabine Schlecht,2 and Wolfgang J. Neubert1*

Department of Molecular Virology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany,1 Bristol-Myers Squibb GmbH & Co. KGaA, Sapporobogen 6-8, 80637 München, Germany2

Received 29 April 2007/ Accepted 30 August 2007

Among the members of the paramyxovirus family, the transcription process and the components involved have been studied under in vitro conditions thus far. Here, we reexamined the function of the viral RNA-dependent RNA polymerase through infection studies with Sendai virus (SeV) N and P deletion ({Delta}) mutants. To elucidate solely transcription-specific processes, all virus mutants also were rendered deficient in genome replication. Using mutant SeV {Delta}P, the earlier suspected supplemental role of P protein was clearly demonstrated to be essential during viral gene expression. Moreover, when SeV {Delta}N or {Delta}N P{Delta}2-77 (with the 5' end of the P gene deleted) mutant was used for infections, a completely unexpected new and essential role for N protein was discovered for viral gene expression. In the early phases of an infection and in the absence of de novo viral protein synthesis, primary transcription occurs at hardly detectable levels. In contrast, if newly synthesized N protein is present, primary viral transcription reaches normal levels. From our data, we conclude that de novo synthesis of SeV N and P proteins is a key step for viral gene expression that facilitates the transition from preliminary to normal primary transcriptional activity.

* Corresponding author. Mailing address: Max-Planck-Institute of Biochemistry, Department of Molecular Virology, Am Klopferspitz 18, 82152 Martinsried, Germany. Phone: 49 89 8578 2268. Fax: 49 89 8578 2909. E-mail: neubert{at}biochem.mpg.de

{triangledown} Published ahead of print on 12 September 2007.

Journal of Virology, December 2007, p. 13835-13844, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.00914-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Murphy, A. M., Grdzelishvili, V. Z. (2009). Identification of Sendai Virus L Protein Amino Acid Residues Affecting Viral mRNA Cap Methylation. J. Virol. 83: 1669-1681 [Abstract] [Full Text]  


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