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Journal of Virology, December 2007, p. 12881-12888, Vol. 81, No. 23
0022-538X/07/$08.00+0     doi:10.1128/JVI.00913-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

HC-Pro Protein of Potato Virus Y Can Interact with Three Arabidopsis 20S Proteasome Subunits In Planta{triangledown}

Yongsheng Jin,1,{dagger} Dongyuan Ma,1,{dagger} Jiangli Dong,1 Jingchen Jin,2 Daofeng Li,1 Changwang Deng,1 and Tao Wang1*

State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing 100094, People's Republic of China,1 Henan Agricultural University, Zhengzhou 450002, People's Republic of China2

Received 28 April 2007/ Accepted 16 September 2007

The multifunctional protein helper component proteinase (HC-Pro) is thought to interfere with the activity of the 20S proteasome; however, no sites of interaction have been identified for either protein. Here, we first show that the Potato virus Y (PVY) HC-Pro protein can interact with three Arabidopsis 20S proteasome subunits (PAA, PBB, and PBE), using a yeast two-hybrid system and the bimolecular fluorescence complement assay. In addition, yeast two-hybrid analysis of the interaction between several mutant subunits of the 20S proteasome and PVY HC-Pro confirmed that residues 81 to 140 of PAA, 1 to 80 of PBB, and 160 to 274 of PBE are necessary for binding PAA, PBB, and PBE to PVY HC-Pro, respectively. Deletion mutant analysis of PVY HC-Pro showed that the N terminus (residues 1 to 97) is necessary for its interaction with three Arabidopsis 20S proteasome subunits. The ability of HC-Pro to interact and interfere with the activity of the 20S proteasome may help explain the molecular basis of its multifunctional character.

* Corresponding author. Mailing address: State Key Laboratory of Agro-biotechnology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, PR China. Phone and fax: 86-10-62733969. E-mail: wangt{at}cau.edu.cn

{triangledown} Published ahead of print on 26 September 2007.

{dagger} Dongyuan Ma and Yongsheng Jin contributed equally to this work.

Journal of Virology, December 2007, p. 12881-12888, Vol. 81, No. 23
0022-538X/07/$08.00+0     doi:10.1128/JVI.00913-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cheng, Y.-Q., Liu, Z.-M., Xu, J., Zhou, T., Wang, M., Chen, Y.-T., Li, H.-F., Fan, Z.-F. (2008). HC-Pro protein of sugar cane mosaic virus interacts specifically with maize ferredoxin-5 in vitro and in planta. J. Gen. Virol. 89: 2046-2054 [Abstract] [Full Text]  


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