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Identification of the DNA Sequence Interacting with Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 1

Korea Basic Science Institute, Gwangju 500-757, Korea,¹ Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 301-832, Korea,² Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea³

Received 16 March 2007/ Accepted 4 September 2007

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma. The open reading frame (K9) of KSHV encodes viral interferon regulatory factor 1 (vIRF1), which functions as a repressor of interferon-mediated signal transduction. The amino-terminal region of vIRF1 displays significant homology to the DNA-binding domain of cellular interferon regulatory factors, supporting the theory that the protein interacts with specific DNA sequences. Here, we identify the consensus sequence of vIRF1-binding sites from a pool of random oligonucleotides. Moreover, our data show that vIRF1 interacts with the K3:viral dihydrofolate reductase:viral interleukin 6 promoter region in the KSHV genome.

Published ahead of print on 12 September 2007.