This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Taylor, M. P.
Right arrow Articles by Kirkegaard, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taylor, M. P.
Right arrow Articles by Kirkegaard, K.

 Previous Article  |  Next Article 

Journal of Virology, November 2007, p. 12543-12553, Vol. 81, No. 22
0022-538X/07/$08.00+0     doi:10.1128/JVI.00755-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Modification of Cellular Autophagy Protein LC3 by Poliovirus{triangledown}

Matthew P. Taylor and Karla Kirkegaard*

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305

Received 7 April 2007/ Accepted 27 August 2007

Poliovirus infection remodels intracellular membranes, creating a large number of membranous vesicles on which viral RNA replication occurs. Poliovirus-induced vesicles display hallmarks of cellular autophagosomes, including delimiting double membranes surrounding the cytosolic lumen, acquisition of the endosomal marker LAMP-1, and recruitment of the 18-kDa host protein LC3. Autophagy results in the covalent lipidation of LC3, conferring the property of membrane association to this previously microtubule-associated protein and providing a biochemical marker for the induction of autophagy. Here, we report that a similar modification of LC3 occurs both during poliovirus infection and following expression of a single viral protein, a stable precursor termed 2BC. Therefore, one of the early steps in cellular autophagy, LC3 modification, can be genetically separated from the induction of double-membraned vesicles that contain the modified LC3, which requires both viral proteins 2BC and 3A. The existence of viral inducers that promote a distinct aspect of the formation of autophagosome-like membranes both facilitates the dissection of this cellular process and supports the hypothesis that this branch of the innate immune response is directly subverted by poliovirus.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, 299 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305. Phone: (650) 498-7075. Fax: (650) 498-7147. E-mail: karlak{at}stanford.edu

{triangledown} Published ahead of print on 5 September 2007.

Journal of Virology, November 2007, p. 12543-12553, Vol. 81, No. 22
0022-538X/07/$08.00+0     doi:10.1128/JVI.00755-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cianciola, N. L., Carlin, C. R. (2009). Adenovirus RID-{alpha} activates an autonomous cholesterol regulatory mechanism that rescues defects linked to Niemann-Pick disease type C. JCB 187: 537-552 [Abstract] [Full Text]  
  • Taylor, M. P., Burgon, T. B., Kirkegaard, K., Jackson, W. T. (2009). Role of Microtubules in Extracellular Release of Poliovirus. J. Virol. 83: 6599-6609 [Abstract] [Full Text]  
  • Armer, H., Moffat, K., Wileman, T., Belsham, G. J., Jackson, T., Duprex, W. P., Ryan, M., Monaghan, P. (2008). Foot-and-Mouth Disease Virus, but Not Bovine Enterovirus, Targets the Host Cell Cytoskeleton via the Nonstructural Protein 3Cpro. J. Virol. 82: 10556-10566 [Abstract] [Full Text]  
  • Xavier, R. J, Huett, A., Rioux, J. D (2008). Autophagy as an important process in gut homeostasis and Crohn's disease pathogenesis. Gut 57: 717-720 [Full Text]  
  • Ait-Goughoulte, M., Kanda, T., Meyer, K., Ryerse, J. S., Ray, R. B., Ray, R. (2008). Hepatitis C Virus Genotype 1a Growth and Induction of Autophagy. J. Virol. 82: 2241-2249 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»