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Journal of Virology, November 2007, p. 12348-12359, Vol. 81, No. 22
0022-538X/07/$08.00+0     doi:10.1128/JVI.01177-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Morphogenesis of a Highly Replicative EGFPVP22 Recombinant Marek's Disease Virus in Cell Culture{triangledown}

C. Denesvre,1* C. Blondeau,1 M. Lemesle,3 Y. Le Vern,2 D. Vautherot,1 P. Roingeard,3 and J. F. Vautherot1

Laboratoire de Virologie Moléculaire, INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly 37380, France,1 Service Commun de Cytométrie, Centre INRA de Tours, 37380 Nouzilly, France,2 Université François Rabelais, INSERM ERI 19, Faculté de Médecine & CHRU, 10 boulevard Tonnelé, 37032 Tours, France3

Received 30 May 2007/ Accepted 4 September 2007

Marek's disease virus (MDV) is an alphaherpesvirus for which infection is strictly cell associated in permissive cell culture systems. In contrast to most other alphaherpesviruses, no comprehensive ultrastructural study has been published to date describing the different stages of MDV morphogenesis. To circumvent problems linked to nonsynchronized infection and low infectivity titers, we generated a recombinant MDV expressing an enhanced green fluorescent protein fused to VP22, a major tegument protein that is not implicated in virion morphogenesis. Growth of this recombinant virus in cell culture was decreased threefold compared to that of the parental Bac20 virus, but this mutant was still highly replicative. The recombinant virus allowed us to select infected cells by cell-sorting cytometry at late stages of infection for subsequent transmission electron microscopy analysis. Under these conditions, all of the stages of assembly and virion morphogenesis could be observed except extracellular enveloped virions, even at the cell surface. We observed 10-fold fewer naked cytoplasmic capsids than nuclear capsids, and intracellular enveloped virions were very rare. The partial envelopment of capsids in the cytoplasm supports the hypothesis of the acquisition of the final envelope in this cellular compartment. We demonstrate for the first time that, compared to other alphaherpesviruses, MDV seems deficient in three crucial steps of viral morphogenesis, i.e., release from the nucleus, secondary envelopment, and the exocytosis process. The discrepancy between the efficiency with which this MDV mutant spreads in cell culture and the relatively inefficient process of its envelopment and virion release raises the question of the MDV cell-to-cell spreading mechanism.

* Corresponding author. Mailing address: Laboratoire Virologie Moléculaire, INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly F-37380, France. Phone: (33) 2 47 42 76 19. Fax: (33) 2 47 42 77 74. E-mail: denesvre{at}tours.inra.fr

{triangledown} Published ahead of print on 12 September 2007.

Journal of Virology, November 2007, p. 12348-12359, Vol. 81, No. 22
0022-538X/07/$08.00+0     doi:10.1128/JVI.01177-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Blondeau, C., Marc, D., Courvoisier, K., Vautherot, J.-F., Denesvre, C. (2008). Functional Homologies between Avian and Human Alphaherpesvirus VP22 Proteins in Cell-to-Cell Spreading as Revealed by a New cis-Complementation Assay. J. Virol. 82: 9278-9282 [Abstract] [Full Text]  


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