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Journal of Virology, November 2007, p. 12077-12079, Vol. 81, No. 21
0022-538X/07/$08.00+0     doi:10.1128/JVI.01133-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus Mutants with Multiple Substitutions Affecting DNA Binding of UL42 Are Impaired for Viral Replication and DNA Synthesis{triangledown}

Changying Jiang,1 Ying T. Hwang,1 Guangliang Wang,1 John C. W. Randell,2,{dagger} Donald M. Coen,2 and Charles B. C. Hwang1*

Department of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, New York 13210,1 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 021152

Received 24 May 2007/ Accepted 13 August 2007

Herpes simplex virus mutants with single substitutions that decrease DNA binding by the DNA polymerase processivity subunit UL42 are only modestly impaired for viral replication. In this study, recombinant viruses harboring two or four of these mutations were constructed. The more substitutions, the more severe the defects in viral replication and DNA synthesis, suggesting that DNA binding by UL42 is important for these processes.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, NY 13210. Phone: (315) 464-8739. Fax: (315) 464-4417. E-mail: hwangc{at}upstate.edu

{triangledown} Published ahead of print on 22 August 2007.

{dagger} Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.

Journal of Virology, November 2007, p. 12077-12079, Vol. 81, No. 21
0022-538X/07/$08.00+0     doi:10.1128/JVI.01133-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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