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Journal of Virology, November 2007, p. 11790-11797, Vol. 81, No. 21
0022-538X/07/$08.00+0     doi:10.1128/JVI.01113-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Capsid and Tegument of the Alphaherpesviruses Are Linked by an Interaction between the UL25 and VP1/2 Proteins{triangledown}

Kelly Elizabeth Coller, Joy I-Hsuan Lee, Aki Ueda, and Gregory Allan Smith*

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611

Received 22 May 2007/ Accepted 13 August 2007

How alphaherpesvirus capsids acquire tegument proteins remains a key question in viral assembly. Using pseudorabies virus (PRV), we have previously shown that the 62 carboxy-terminal amino acids of the VP1/2 large tegument protein are essential for viral propagation and when transiently expressed as a fusion to green fluorescent protein relocalize to nuclear capsid assemblons following viral infection. Here, we show that localization of the VP1/2 capsid-binding domain (VP1/2cbd) into assemblons is conserved in herpes simplex virus type 1 (HSV-1) and that this recruitment is specifically on capsids. Using a mutant virus screen, we find that the protein product of the UL25 gene is essential for VP1/2cbd association with capsids. An interaction between UL25 and VP1/2 was corroborated by coimmunoprecipitation from cells transiently expressing either HSV-1 or PRV proteins. Taken together, these findings suggest that the essential function of the VP1/2 carboxy terminus is to anchor the VP1/2 tegument protein to capsids. Furthermore, UL25 encodes a multifunctional capsid protein involved in not only encapsidation, as previously described, but also tegumentation.

* Corresponding author. Mailing address: Department of Microbiology-Immunology, Ward Bldg., Room 10-105, Northwestern University Feinberg School of Medicine, Chicago, IL 60611. Phone: (312) 503-3745. Fax: (312) 503-1339. E-mail: g-smith3{at}northwestern.edu

{triangledown} Published ahead of print on 22 August 2007.

Journal of Virology, November 2007, p. 11790-11797, Vol. 81, No. 21
0022-538X/07/$08.00+0     doi:10.1128/JVI.01113-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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