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Journal of Virology, October 2007, p. 11526-11531, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.01041-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Probing the Flavivirus Membrane Fusion Mechanism by Using Monoclonal Antibodies{triangledown}

Karin Stiasny,* Samantha Brandler,{dagger} Christian Kössl, and Franz X. Heinz

Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT-1095 Vienna, Austria

Received 14 May 2007/ Accepted 20 July 2007

In this study, we investigated in a flavivirus model (tick-borne encephalitis virus) the mechanisms of fusion inhibition by monoclonal antibodies directed to the different domains of the fusion protein (E) and to different sites within each of the domains by using in vitro fusion assays. Our data indicate that, depending on the location of their binding sites, the monoclonal antibodies impaired early or late stages of the fusion process, by blocking the initial interaction with the target membrane or by interfering with the proper formation of the postfusion structure of E, respectively. These data provide new insights into the mechanisms of flavivirus fusion inhibition by antibodies and their possible contribution to virus neutralization.

* Corresponding author. Mailing address: Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, AT-1095, Vienna, Austria. Phone: 43-1-40490, ext. 79505. Fax: 43-1-40490, ext. 9795. E-mail: karin.stiasny{at}meduniwien.ac.at

{triangledown} Published ahead of print on 1 August 2007.

{dagger} Present address: Viral Genomics and Vaccination Laboratory, Institut Pasteur, CNRS-URA3015, 28 rue du Dr Roux, 75015 Paris, France.

Journal of Virology, October 2007, p. 11526-11531, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.01041-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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