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Journal of Virology, October 2007, p. 11381-11391, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.00767-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Cytoplasmic Tails of Uukuniemi Virus (Bunyaviridae) G_N and G_C Glycoproteins Are Important for Intracellular Targeting and the Budding of Virus-Like Particles

Ludwig Institute for Cancer Research, Stockholm Branch, Karolinska Institute, Box 240, SE-17177 Stockholm, Sweden,¹ University of Texas Medical Branch, Department of Pathology, 301 University Boulevard, Galveston, Texas 77555-0609²

Received 10 April 2007/ Accepted 24 July 2007

Functional motifs within the cytoplasmic tails of the two glycoproteins G_N and G_C of Uukuniemi virus (UUK) (Bunyaviridae family) were identified with the help of our recently developed virus-like particle (VLP) system for UUK virus (A. K. Overby, V. Popov, E. P. Neve, and R. F. Pettersson, J. Virol. 80:10428-10435, 2006). We previously reported that information necessary for the packaging of ribonucleoproteins into VLPs is located within the G_N cytoplasmic tail (A. K. Overby, R. F. Pettersson, and E. P. Neve, J. Virol. 81:3198-3205, 2007). The G_N glycoprotein cytoplasmic tail specifically interacts with the ribonucleoproteins and is critical for genome packaging. In addition, two other regions in the G_N cytoplasmic tail, encompassing residues 21 to 25 and 46 to 50, were shown to be important for particle generation and release. By the introduction of point mutations within these two regions, we demonstrate that leucines at positions 23 and 24 are crucial for the initiation of VLP budding, while leucine 46, glutamate 47, and leucine 50 are important for efficient exit from the endoplasmic reticulum and subsequent transport to the Golgi complex. We found that budding and particle generation are highly dependent on the intracellular localization of both glycoproteins. The short cytoplasmic tail of UUK G_C contains a lysine at position –3 from the C terminus that is highly conserved among members of the Phlebovirus, Hantavirus, and Orthobunyavirus genera. Mutating this single amino acid residue in G_C resulted in the mislocalization of not only G_C but also G_N to the plasma membrane, and VLP generation was compromised in cells expressing this mutant. Together, these results demonstrate that the cytoplasmic tails of both G_N and G_C contain specific information necessary for efficient virus particle generation.

Published ahead of print on 1 August 2007.

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