JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JVI.00910-07v1
81/20/11282    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Crescenzo-Chaigne, B.
Right arrow Articles by van der Werf, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Crescenzo-Chaigne, B.
Right arrow Articles by van der Werf, S.

 Previous Article  |  Next Article 

Journal of Virology, October 2007, p. 11282-11289, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.00910-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Rescue of Influenza C Virus from Recombinant DNA{triangledown}

Bernadette Crescenzo-Chaigne and Sylvie van der Werf*

Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, EA 302 Université Paris Diderot, Institut Pasteur, Paris, France

Received 28 April 2007/ Accepted 30 July 2007

The rescue of influenza viruses by reverse genetics has been described only for the influenza A and B viruses. Based on a similar approach, we developed a reverse-genetics system that allows the production of influenza C viruses entirely from cloned cDNA. The complete sequences of the 3' and 5' noncoding regions of type C influenza virus C/Johannesburg/1/66 necessary for the cloning of the cDNA were determined for the seven genomic segments. Human embryonic kidney cells (293T) were transfected simultaneously with seven plasmids that direct the synthesis of each of the seven viral RNA segments of the C/JHB/1/66 virus under the control of the human RNA polymerase I promoter and with four plasmids encoding the viral nucleoprotein and the PB2, PB1, and P3 proteins of the viral polymerase complex. This strategy yielded between 103 and 104 PFU of virus per ml of supernatant at 8 to 10 days posttransfection. Additional viruses with substitutions introduced in the hemagglutinin-esterase-fusion protein were successfully produced by this method, and their growth phenotype was evaluated. This efficient system, which does not require helper virus infection, should be useful in viral mutagenesis studies and for generation of expression vectors from type C influenza virus.

* Corresponding author. Mailing address: Unité de Génétique Moléculaire des Virus Respiratoires, URA CNRS 1966, EA 302 Université Paris Diderot, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33-1-45-68-87-22. Fax: 33-1-40-61-32-41. E-mail: svdwerf{at}pasteur.fr

{triangledown} Published ahead of print on 8 August 2007.

Journal of Virology, October 2007, p. 11282-11289, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.00910-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2007 by the American Society for Microbiology. All rights reserved.