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Journal of Virology, October 2007, p. 10897-10904, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.00959-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Assembly of Hepatitis B Virus Envelope Proteins onto a Lentivirus Pseudotype That Infects Primary Human Hepatocytes{triangledown}

Ning Chai, Ho Eun Chang, Emmanuelle Nicolas, Severin Gudima, Jinhong Chang, and John Taylor*

Fox Chase Cancer Center, Philadelphia, Pennsylvania

Received 3 May 2007/ Accepted 21 July 2007

This study demonstrates that the envelope proteins of hepatitis B virus (HBV) could be incorporated into the lipid membrane of lentivirus pseudotype particles. The assembly procedure was initiated by the transfection of 293T cells with three plasmids: (i) a human immunodeficiency virus (HIV) packaging construct, (ii) a transfer plasmid expressing a reporter gene, and (iii) a plasmid expressing large (L), middle (M), and small (S) HBV envelope proteins. After 2 days, hepatitis B surface antigen and the antigenic forms of L, M, and S were detected at the cell surface by flow cytometry. Also, virus particles that were able to infect cultured primary human hepatocytes (PHH) were released. Under optimal conditions, 50% of PHH could be infected. In addition, the susceptibility of PHH and the resistance of other cell types to the pseudotype particles were similar to those observed for HBV and hepatitis delta virus (HDV), which shares the same L, M, and S. Furthermore, the infection of PHH by the pseudotype was sensitive to known inhibitors of HBV and HDV entry. These findings of specific and efficient infection of hepatocytes could be applicable to liver-specific gene therapy and may help clarify the attachment and entry mechanism used by HBV and HDV.

* Corresponding author. Mailing address: Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111-2497. Phone: (215) 728-2436. Fax: (215) 728-3105. E-mail: john.taylor{at}fccc.edu

{triangledown} Published ahead of print on 1 August 2007.

Journal of Virology, October 2007, p. 10897-10904, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.00959-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Le Duff, Y., Blanchet, M., Sureau, C. (2009). The Pre-S1 and Antigenic Loop Infectivity Determinants of the Hepatitis B Virus Envelope Proteins Are Functionally Independent. J. Virol. 83: 12443-12451 [Abstract] [Full Text]  
  • Chai, N., Chang, H. E., Nicolas, E., Han, Z., Jarnik, M., Taylor, J. (2008). Properties of Subviral Particles of Hepatitis B Virus. J. Virol. 82: 7812-7817 [Abstract] [Full Text]  


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