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Journal of Virology, October 2007, p. 10890-10896, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.00807-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Latent Infection of a New Alphanodavirus in an Insect Cell Line{triangledown}

Tian-Cheng Li,1* Paul D. Scotti,2 Tatsuo Miyamura,1 and Naokazu Takeda1

Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-Murayama, Tokyo 208-0011, Japan,1 Waiatarua, Auckland, New Zealand2

Received 14 April 2007/ Accepted 26 July 2007

Insect BTI-TN-5B1-4 (Tn5) cells have been used extensively with recombinant baculoviruses to express foreign genes. When a recombinant baculovirus containing the hepatitis E virus capsid protein gene was used to infect Tn5 cells, unknown virus particles in addition to the anticipated hepatitis E virus-like particles were produced in the infected cells. The unknown virus particles were 35 nm in diameter and contained RNA that was highly homologous to full-length RNA1 (3,107 bp) and RNA2 (1,383 bp) genomic RNAs of flock house virus. Surprisingly, both RNAs seen in these induced nodavirus particles could be amplified from commercially available Tn5 cells without infection with or induction by a baculovirus. The nucleotide sequences from the purified nodavirus particles and the normal Tn5 cells were identical, demonstrating that the Tn5 cells themselves were latently infected with a nodavirus. However, the generation of nodavirus particles was significantly stimulated by infection with recombinant baculoviruses. Phylogenetic analysis suggested that this new nodavirus belongs to the genus Alphanodavirus in the family Nodaviridae.

* Corresponding author. Mailing address: Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-Murayama, Tokyo 208-0011, Japan. Phone: (81) 42-561-0771. Fax: (81) 42-561-4729. E-mail: litc{at}nih.go.jp

{triangledown} Published ahead of print on 8 August 2007.

Journal of Virology, October 2007, p. 10890-10896, Vol. 81, No. 20
0022-538X/07/$08.00+0     doi:10.1128/JVI.00807-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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