This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johansson, C.
Right arrow Articles by Arnberg, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johansson, C.
Right arrow Articles by Arnberg, N.

 Previous Article  |  Next Article 

Journal of Virology, January 2007, p. 954-963, Vol. 81, No. 2
0022-538X/07/$08.00+0     doi:10.1128/JVI.01995-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Adenoviruses Use Lactoferrin as a Bridge for CAR-Independent Binding to and Infection of Epithelial Cells{triangledown}

Cecilia Johansson,1 Mari Jonsson,1 Marko Marttila,1 David Persson,1 Xiao-Long Fan,2 Johan Skog,1 Lars Frängsmyr,3,4 Göran Wadell,1 and Niklas Arnberg1*

Departments of Virology,1 Anatomy,3 Sports Medicine, Umeå University, Umeå, Sweden,4 Department of Molecular Medicine and Gene Therapy, Lund University, Lund, Sweden2

Received 13 September 2006/ Accepted 24 October 2006

Most adenoviruses bind to the coxsackie- and adenovirus receptor (CAR). Surprisingly, CAR is not expressed apically on polarized cells and is thus not easily available to viruses. Consequently, alternative mechanisms for entry of coxsackievirus and adenovirus into cells have been suggested. We have found that tear fluid promotes adenovirus infection, and we have identified human lactoferrin (HLf) as the tear fluid component responsible for this effect. HLf alone was found to promote binding of adenovirus to epithelial cells in a dose-dependent manner and also infection of epithelial cells by adenovirus. HLf was also found to promote gene delivery from an adenovirus-based vector. The mechanism takes place at the binding stage and functions independently of CAR. Thus, we have identified a novel binding mechanism whereby adenovirus hijacks HLf, a component of the innate immune system, and uses it as a bridge for attachment to host cells.

* Corresponding author. Mailing address: Department of Virology, Umeå University, Umeå SE-90185, Sweden. Phone: 46 90 7858440. Fax: 46 90 129905. E-mail: niklas.arnberg{at}climi.umu.se.

{triangledown} Published ahead of print on 1 November 2006.

Journal of Virology, January 2007, p. 954-963, Vol. 81, No. 2
0022-538X/07/$08.00+0     doi:10.1128/JVI.01995-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Adams, W. C., Bond, E., Havenga, M. J. E., Holterman, L., Goudsmit, J., Karlsson Hedestam, G. B., Koup, R. A., Lore, K. (2009). Adenovirus serotype 5 infects human dendritic cells via a coxsackievirus-adenovirus receptor-independent receptor pathway mediated by lactoferrin and DC-SIGN. J. Gen. Virol. 90: 1600-1610 [Abstract] [Full Text]  
  • Jonsson, M. I., Lenman, A. E., Frangsmyr, L., Nyberg, C., Abdullahi, M., Arnberg, N. (2009). Coagulation Factors IX and X Enhance Binding and Infection of Adenovirus Types 5 and 31 in Human Epithelial Cells. J. Virol. 83: 3816-3825 [Abstract] [Full Text]  
  • Russell, W. C. (2009). Adenoviruses: update on structure and function. J. Gen. Virol. 90: 1-20 [Abstract] [Full Text]  
  • Krautkramer, E., Zeier, M. (2008). Hantavirus Causing Hemorrhagic Fever with Renal Syndrome Enters from the Apical Surface and Requires Decay-Accelerating Factor (DAF/CD55). J. Virol. 82: 4257-4264 [Abstract] [Full Text]  
  • Lore, K., Adams, W. C., Havenga, M., Precopio, M. L., Holterman, L., Goudsmit, J., Koup, R. A. (2007). Myeloid and Plasmacytoid Dendritic Cells Are Susceptible to Recombinant Adenovirus Vectors and Stimulate Polyfunctional Memory T Cell Responses. J. Immunol. 179: 1721-1729 [Abstract] [Full Text]  
  • Parker, A. L., McVey, J. H., Doctor, J. H., Lopez-Franco, O., Waddington, S. N., Havenga, M. J. E., Nicklin, S. A., Baker, A. H. (2007). Influence of Coagulation Factor Zymogens on the Infectivity of Adenoviruses Pseudotyped with Fibers from Subgroup D. J. Virol. 81: 3627-3631 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»