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 Previous Article

Journal of Virology, January 2007, p. 1048-1051, Vol. 81, No. 2
0022-538X/07/$08.00+0     doi:10.1128/JVI.01801-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Efficient Incorporation of Tegument Proteins pUL46, pUL49, and pUS3 into Pseudorabies Virus Particles Depends on the Presence of pUL21{triangledown}

Kathrin Michael, Barbara G. Klupp, Axel Karger, and Thomas C. Mettenleiter*

Institute of Molecular Biology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany

Received 18 August 2006/ Accepted 23 October 2006

The mature virion of the alphaherpesvirus pseudorabies virus (PrV) contains a minimum of 31 structural proteins which are recruited into the virus particle by a network of protein-protein interactions which is only incompletely understood. We show here that deletion of the tegument protein pUL21 resulted in a drastic decrease in the incorporation of the pUL46, pUL49, and pUS3 tegument components into mature virions. Moreover, the attenuated PrV strain Bartha (PrV-Ba), which, among other defects, carries mutations in pUL21, also fails to package pUL46, pUL49, and pUS3 efficiently. By the reconstitution of wild-type pUL21 expression to PrV-Ba and the transfer of mutated PrV-Ba pUL21 into wild-type PrV, we demonstrate that this phenotype is due to the mutated pUL21.

* Corresponding author. Mailing address: Friedrich-Loeffler-Institut, Institute of Molecular Biology, Boddenblick 5A, 17493 Greifswald-Insel Riems, Germany. Phone: 49-38351-7250. Fax: 49-38351-7151. E-mail: thomas.mettenleiter{at}fli.bund.de.

{triangledown} Published ahead of print on 1 November 2006.

Journal of Virology, January 2007, p. 1048-1051, Vol. 81, No. 2
0022-538X/07/$08.00+0     doi:10.1128/JVI.01801-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Curanovic, D., Lyman, M. G., Bou-Abboud, C., Card, J. P., Enquist, L. W. (2009). Repair of the UL21 Locus in Pseudorabies Virus Bartha Enhances the Kinetics of Retrograde, Transneuronal Infection In Vitro and In Vivo. J. Virol. 83: 1173-1183 [Abstract] [Full Text]  


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