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Journal of Virology, January 2007, p. 1022-1026, Vol. 81, No. 2
0022-538X/07/$08.00+0     doi:10.1128/JVI.01944-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Replicase Genes of Murine Coronavirus Strains A59 and JHM Are Interchangeable: Differences in Pathogenesis Map to the 3' One-Third of the Genome{triangledown}

Sonia Navas-Martin,1,2* Maarten Brom,1,{dagger} Ming-Ming Chua,1 Richard Watson,1 Zhaozhu Qiu,1,{ddagger} and Susan R. Weiss1*

Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104,1 Department of Microbiology and Immunology, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, Pennsylvania 191022

Received 6 September 2006/ Accepted 20 October 2006

The important roles of the spike protein and other structural proteins in murine coronavirus (MHV) pathogenesis have been demonstrated; however, the role of the replicase gene remains unexplored. We assessed the influence of the replicase genes of the highly neurovirulent MHV-JHM strain and the hepatotropic and mildly neurovirulent A59 strain in acute infection of the mouse. Analysis of chimeric A59/JHM recombinant viruses indicates that the replicase genes are interchangeable and that it is the 3' end of the genome, encoding the structural proteins, rather than the replicase gene, that determines the pathogenic properties of these chimeras.

* Corresponding author. Mailing address for Sonia Navas-Martin: Department of Microbiology and Immunology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA 19102. Phone: (215) 762-7482. Fax: (215) 762-1955. E-mail: sonia.navas-martin{at}drexelmed.edu. Mailing address for Susan R. Weiss: Department of Microbiology, University of Pennsylvania School of Medicine, 36th Street and Hamilton Walk, Philadelphia, PA 19104-6076. Phone: (215) 898-8013. Fax: (215) 573-4858. E-mail: weisssr{at}mail.med.upenn.edu.

{triangledown} Published ahead of print on 1 November 2006.

{dagger} Present address: Central Animal Laboratory, University Medical Centre St. Radboud, Nijmegen, The Netherlands.

{ddagger} Present address: Microbiology Graduate Program, College of Physicians and Surgeons, Columbia University, New York, NY.

Journal of Virology, January 2007, p. 1022-1026, Vol. 81, No. 2
0022-538X/07/$08.00+0     doi:10.1128/JVI.01944-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Roth-Cross, J. K., Stokes, H., Chang, G., Chua, M. M., Thiel, V., Weiss, S. R., Gorbalenya, A. E., Siddell, S. G. (2009). Organ-Specific Attenuation of Murine Hepatitis Virus Strain A59 by Replacement of Catalytic Residues in the Putative Viral Cyclic Phosphodiesterase ns2. J. Virol. 83: 3743-3753 [Abstract] [Full Text]  


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