This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Finkelstein, D. B.
Right arrow Articles by Naeve, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Finkelstein, D. B.
Right arrow Articles by Naeve, C. W.

 Previous Article  |  Next Article 

Journal of Virology, October 2007, p. 10292-10299, Vol. 81, No. 19
0022-538X/07/$08.00+0     doi:10.1128/JVI.00921-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Persistent Host Markers in Pandemic and H5N1 Influenza Viruses{triangledown}

David B. Finkelstein,1 Suraj Mukatira,1 Perdeep K. Mehta,1 John C. Obenauer,1 Xiaoping Su,1 Robert G. Webster,2 and Clayton W. Naeve1,3*

Hartwell Center for Bioinformatics and Biotechnology, St. Jude Children's Research Hospital, 332 North Lauderdale Street, Memphis, Tennessee 38105-2794,1 Department of Infectious Diseases, St. Jude Children's Research Hospital, 332 North Lauderdale Street, Memphis, Tennessee 38105-2794,2 Department of Pathology, University of Tennessee Health Science Center, Memphis, Tennessee 381053

Received 30 April 2007/ Accepted 13 July 2007

Avian influenza viruses have adapted to human hosts, causing pandemics in humans. The key host-specific amino acid mutations required for an avian influenza virus to function in humans are unknown. Through multiple-sequence alignment and statistical testing of each aligned amino acid, we identified markers that discriminate human influenza viruses from avian influenza viruses. We applied strict thresholds to select only markers which are highly preserved in human influenza virus isolates over time. We found that a subset of these persistent host markers exist in all human pandemic influenza virus sequences from 1918, 1957, and 1968, while others are acquired as the virus becomes a seasonal influenza virus. We also show that human H5N1 influenza viruses are significantly more likely to contain the amino acid predominant in human strains for a few persistent host markers than avian H5N1 influenza viruses. This sporadic enrichment of amino acids present in human-hosted viruses may indicate that some H5N1 viruses have made modest adaptations to their new hosts in the recent past. The markers reported here should be useful in monitoring potential pandemic influenza viruses.

* Corresponding author. Mailing address: Hartwell Center for Bioinformatics and Biotechnology, St. Jude Children's Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105-2794. Phone: (901) 495-3689. Fax: (901) 495-2945. E-mail: clayton.naeve{at}stjude.org

{triangledown} Published ahead of print on 25 July 2007.

Journal of Virology, October 2007, p. 10292-10299, Vol. 81, No. 19
0022-538X/07/$08.00+0     doi:10.1128/JVI.00921-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Holmes, E. C. (2010). Evolution in Health and Medicine Sackler Colloquium: The comparative genomics of viral emergence. Proc. Natl. Acad. Sci. USA 107: 1742-1746 [Abstract] [Full Text]  
  • Shinya, K., Makino, A., Ozawa, M., Kim, J. H., Sakai-Tagawa, Y., Ito, M., Le, Q. M., Kawaoka, Y. (2009). Ostrich Involvement in the Selection of H5N1 Influenza Virus Possessing Mammalian-Type Amino Acids in the PB2 Protein. J. Virol. 83: 13015-13018 [Abstract] [Full Text]  
  • Ghedin, E., Fitch, A., Boyne, A., Griesemer, S., DePasse, J., Bera, J., Zhang, X., Halpin, R. A., Smit, M., Jennings, L., St. George, K., Holmes, E. C., Spiro, D. J. (2009). Mixed Infection and the Genesis of Influenza Virus Diversity. J. Virol. 83: 8832-8841 [Abstract] [Full Text]  
  • Dunham, E. J., Dugan, V. G., Kaser, E. K., Perkins, S. E., Brown, I. H., Holmes, E. C., Taubenberger, J. K. (2009). Different Evolutionary Trajectories of European Avian-Like and Classical Swine H1N1 Influenza A Viruses. J. Virol. 83: 5485-5494 [Abstract] [Full Text]  
  • Le, Q. M., Sakai-Tagawa, Y., Ozawa, M., Ito, M., Kawaoka, Y. (2009). Selection of H5N1 Influenza Virus PB2 during Replication in Humans. J. Virol. 83: 5278-5281 [Abstract] [Full Text]  
  • Hoper, D., Hoffmann, B., Beer, M. (2009). Simple, Sensitive, and Swift Sequencing of Complete H5N1 Avian Influenza Virus Genomes. J. Clin. Microbiol. 47: 674-679 [Abstract] [Full Text]  
  • Bradel-Tretheway, B. G., Kelley, Z., Chakraborty-Sett, S., Takimoto, T., Kim, B., Dewhurst, S. (2008). The human H5N1 influenza A virus polymerase complex is active in vitro over a broad range of temperatures, in contrast to the WSN complex, and this property can be attributed to the PB2 subunit. J. Gen. Virol. 89: 2923-2932 [Abstract] [Full Text]  
  • Monne, I., Fusaro, A., Al-Blowi, M. H., Ismail, M. M., Khan, O. A., Dauphin, G., Tripodi, A., Salviato, A., Marangon, S., Capua, I., Cattoli, G. (2008). Co-circulation of two sublineages of HPAI H5N1 virus in the Kingdom of Saudi Arabia with unique molecular signatures suggesting separate introductions into the commercial poultry and falconry sectors. J. Gen. Virol. 89: 2691-2697 [Abstract] [Full Text]  
  • Hale, B. G., Randall, R. E., Ortin, J., Jackson, D. (2008). The multifunctional NS1 protein of influenza A viruses. J. Gen. Virol. 89: 2359-2376 [Abstract] [Full Text]  
  • Roy, P. (2008). Bluetongue virus: dissection of the polymerase complex. J. Gen. Virol. 89: 1789-1804 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»