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Journal of Virology, September 2007, p. 9870-9877, Vol. 81, No. 18
0022-538X/07/$08.00+0     doi:10.1128/JVI.00001-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The ORF49 Protein of Murine Gammaherpesvirus 68 Cooperates with RTA in Regulating Virus Replication{triangledown}

Sangmi Lee,1,2 Hye-Jeong Cho,1 Jung-Jin Park,1 Yong-Sun Kim,2 Seungmin Hwang,3 Ren Sun,3 and Moon Jung Song1*

Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713,1 Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea,2 Department of Molecular and Medical Pharmacology, University of California at Los Angeles, Los Angeles, California 900953

Received 2 January 2007/ Accepted 28 June 2007

Our functional mapping study of murine gammaherpesvirus 68 (MHV-68, or {gamma}HV-68) revealed that a mutant harboring a transposon at the ORF49 locus (ORF49null) evidenced a highly attenuated in vitro growth. ORF49 resides adjacent to and in an opposite direction from RTA, the primary switch of the gammaherpesvirus life cycle. A FLAG-tagged ORF49 protein was able to transcomplement ORF49null, and a revertant of ORF49null restored its attenuated growth to a level comparable to that of the wild type. The FLAG-tagged ORF49 protein promoted the ability of RTA to activate downstream target promoters and enhanced virus replication from the ORF50null virus in the presence of RTA. Furthermore, ORF49 enhanced wild-type virus replication by increasing the RTA transcript levels. Our data indicate that ORF49 may perform an important function in MHV-68 replication in cooperation with RTA.

* Corresponding author. Mailing address: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea. Phone: 82-2-3290-3019. Fax: 82-2-3291-3012. E-mail: moonsong{at}korea.ac.kr

{triangledown} Published ahead of print on 18 July 2007.

Journal of Virology, September 2007, p. 9870-9877, Vol. 81, No. 18
0022-538X/07/$08.00+0     doi:10.1128/JVI.00001-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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