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Journal of Virology, September 2007, p. 9759-9768, Vol. 81, No. 18
0022-538X/07/$08.00+0     doi:10.1128/JVI.00844-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Interactions between Brome Mosaic Virus RNAs and Cytoplasmic Processing Bodies{triangledown}

Carla J. Beckham,1 Heather R. Light,2 T. Amar Nissan,5 Paul Ahlquist,3,4 Roy Parker,4,5* and Amine Noueiry2,{dagger}

Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona,1 Davis College of Agriculture, Forestry and Consumer Science, West Virginia University, Morgantown, West Virginia,2 Institute for Molecular Virology, University of Wisconsin—Madison, Madison, Wisconsin,3 Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona,4 Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona5

Received 20 April 2007/ Accepted 22 June 2007

Cytoplasmic processing bodies are sites where nontranslating mRNAs accumulate for different fates, including decapping and degradation, storage, or returning to translation. Previous work has also shown that the Lsm1-7p complex, Dhh1p, and Pat1p, which are all components of P bodies, are required for translation and subsequent recruitment to replication of the plant virus brome mosaic virus (BMV) genomic RNAs when replication is reproduced in yeast cells. To better understand the role of P bodies in BMV replication, we examined the subcellular locations of BMV RNAs in yeast cells. We observed that BMV genomic RNA2 and RNA3 accumulated in P bodies in a manner dependent on cis-acting RNA replication signals, which also directed nonviral RNAs to P bodies. Furthermore, the viral RNA-dependent RNA polymerase coimmunoprecipitates and shows partial colocalization with the P-body component Lsm1p. These observations suggest that the accumulation of BMV RNAs in P bodies may be an important step in RNA replication complex assembly for BMV, and possibly for other positive-strand RNA viruses.

* Corresponding author. Mailing address: Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, The University of Arizona, Tucson, AZ 85721-0206. Phone: (520) 621-9347. Fax: (520) 621-4524. E-mail: rrparker{at}u.arizona.edu

{triangledown} Published ahead of print on 3 July 2007.

{dagger} Present address: Apath, LLC, St. Louis, MO.

Journal of Virology, September 2007, p. 9759-9768, Vol. 81, No. 18
0022-538X/07/$08.00+0     doi:10.1128/JVI.00844-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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